Reviewed, UniProtKB/Swiss-Prot Q9VP61 (ACSA_DROME)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme
    Acetyl-CoA synthetase
      Short name=AceCS
      Short name=ACS

Gene names

Name:	AcCoAS
ORF Names:	CG9390

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates acetate so that it can be used for lipid synthesis or for energy generation. UniProtKB Q9NR19
Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. UniProtKB Q9NR19
Subcellular location	Cytoplasm. UniProtKB Q9NR19
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Non-traceable author statement. Source: UniProtKB
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VP61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9VP61-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	Acetyl-coenzyme A synthetase		PRO_0000208425
Natural variations
	Alternative sequence	1 – 146	146	Missing in isoform B.		VSP_008310
Experimental info
	Sequence conflict	227	1	C → S in CAA86738. Ref.1
	Sequence conflict	326	1	A → G in AAF51695. Ref.2
	Sequence conflict	326	1	A → G in AAF51696. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CE24364755CDBFFC	FASTA	670	75,960
	10 20 30 40 50 60 MPAEKSIYDP NPAISQNAYI SSFEEYQKFY QESLDNPAEF WSRVAKQFHW ETPADQDKFL 70 80 90 100 110 120 KYNFNISKGP ISIKWMEGAS TNLCYNLLDR NVRNGLGDQI AYYWEGNHPD DYSRGLTYRK 130 140 150 160 170 180 LLEEVCRFAN VLKDHGIRKG DRVSIYMPMI LELPIAMLAC ARIGAVHSIV FAGFSPDSLA 190 200 210 220 230 240 ERMFDCKAKL LITADGAWRG EKPLYLKALC DTALEKVEEM GHSVEKCIVV SHLKRVTPCQ 250 260 270 280 290 300 PDHVEEEIPW TDDRDYWWHE EMEDKEPACY PEWMDAEDPL FMLYTSGSTG KPKGVLHTTA 310 320 330 340 350 360 GYLLYAATTF KIVFDYKPGD IYWCTADVGW ITGHTYVVYG PLANGATSVI FEGTPFFPGN 370 380 390 400 410 420 DRYWSVIDKY KVTQFYTAPT AIRALMKFGE GPVLKHNLSG LKVLGSVGEP INPEAWLWYY 430 440 450 460 470 480 KYIGKEQCSI VDTFWQTETG GHVITPLPGA TPMKPGSASF PFFGVKPTLL DECGIEIKGE 490 500 510 520 530 540 GEGYLVFSQP WPGMMRTLYN NHERFEDTYF SKFPGYYCTG DGARRDADGY LWITGRVDDM 550 560 570 580 590 600 LNVSGHLMST AEVESVLTEH PRVAESAVVS RPHPVKGECL YCFITPNENE VFDQKLISDL 610 620 630 640 650 660 KKMVRERIGP FAMPDVIQNA PGLPKTRSGK IMRRVLRKIA VNDRNVGDTS TLADEQIVEQ 670 LFANRPVEAK
	Isoform B. Checksum: 63D8E2976CC09CAA	FASTA	524	58,829
	10 20 30 40 50 60 MPMILELPIA MLACARIGAV HSIVFAGFSP DSLAERMFDC KAKLLITADG AWRGEKPLYL 70 80 90 100 110 120 KALCDTALEK VEEMGHSVEK CIVVSHLKRV TPCQPDHVEE EIPWTDDRDY WWHEEMEDKE 130 140 150 160 170 180 PACYPEWMDA EDPLFMLYTS GSTGKPKGVL HTTAGYLLYA ATTFKIVFDY KPGDIYWCTA 190 200 210 220 230 240 DVGWITGHTY VVYGPLANGA TSVIFEGTPF FPGNDRYWSV IDKYKVTQFY TAPTAIRALM 250 260 270 280 290 300 KFGEGPVLKH NLSGLKVLGS VGEPINPEAW LWYYKYIGKE QCSIVDTFWQ TETGGHVITP 310 320 330 340 350 360 LPGATPMKPG SASFPFFGVK PTLLDECGIE IKGEGEGYLV FSQPWPGMMR TLYNNHERFE 370 380 390 400 410 420 DTYFSKFPGY YCTGDGARRD ADGYLWITGR VDDMLNVSGH LMSTAEVESV LTEHPRVAES 430 440 450 460 470 480 AVVSRPHPVK GECLYCFITP NENEVFDQKL ISDLKKMVRE RIGPFAMPDV IQNAPGLPKT 490 500 510 520 RSGKIMRRVL RKIAVNDRNV GDTSTLADEQ IVEQLFANRP VEAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Drosophila melanogaster acetyl-CoA-synthetase gene." Russell S.R., Heimbeck G.M., Carpenter A.T., Ashburner M. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B). Strain: Berkeley. Tissue: Embryo and Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46786 mRNA. Translation: CAA86738.1. Sequence problems. AE014296 Genomic DNA. Translation: AAF51695.2. AE014296 Genomic DNA. Translation: AAF51696.3. AY089540 mRNA. Translation: AAL90278.1. BT001456 mRNA. Translation: AAN71211.1.
PIR	S52154.
RefSeq	NP_524196.2. NP_730611.1.
UniGene	Dm.7388
3D structure databases
HSSP	HSSP built from PDB template 1PG4 based on UniProtKB Q8ZKF6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VP61. 2 interactions.
STRING	Q9VP61.
Proteomic databases
PRIDE	Q9VP61.
Genome annotation databases
Ensembl	FBtr0078413; FBpp0078067; FBgn0012034; Drosophila melanogaster. [Genome view]
GeneID	40348.
Organism-specific databases
CTD	40348.
FlyBase	FBgn0012034. AcCoAS.
Phylogenomic databases
HOGENOM	Q9VP61.
Enzyme and pathway databases
BRENDA	6.2.1.1. 48.
Gene expression databases
Bgee	Q9VP61.
GermOnline	CG9390. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	818306.

Entry information

Entry name

ACSA_DROME

Accession

Primary (citable) accession number: Q9VP61
Secondary accession number(s): Q24226, Q8IH30, Q9VP60

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project