Reviewed, UniProtKB/Swiss-Prot Q9H8T0 (AKTIP_HUMAN)

Names and origin

Protein names

Recommended name:
    AKT-interacting protein
Alternative name(s):
    Fused toes protein homolog
    Ft1

Gene names

Name:	AKTIP
Synonyms:	FTS

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Ref.6 Ref.7
Subunit structure	Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Also interacts with AKT1. Ref.6
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein. Ref.6
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily.
Caution	Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity.

Ontologies

Keywords
Biological process	Apoptosis Protein transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW early endosome to late endosome transport Ref.7 Inferred from mutant phenotype. Source: UniProtKB endosome organization Ref.7 Inferred from mutant phenotype. Source: UniProtKB endosome to lysosome transport Ref.7 Inferred from mutant phenotype. Source: UniProtKB lysosome organization Ref.7 Inferred from mutant phenotype. Source: UniProtKB post-translational protein modification Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	FHF complex Ref.7 Inferred from direct assay. Source: UniProtKB extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.7 Inferred from physical interaction. Source: UniProtKB small conjugating protein ligase activity Ref.7 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DCTN1	Q14203	1	EBI-711399,EBI-724352
HDLBP	Q00341	1	EBI-711399,EBI-1049478
HOOK1	Q9UJC3	1	EBI-711399,EBI-746704
PDS5A	Q29RF7	1	EBI-711399,EBI-1175454
TIMM50	Q3ZCQ8	1	EBI-711399,EBI-355175

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H8T0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H8T0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: Q → QK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	AKT-interacting protein		PRO_0000082609
Natural variations
	Alternative sequence	256	1	Q → QK in isoform 2.		VSP_037631
Experimental info
	Mutagenesis	106 – 107	2	WF → AA: Impairs interaction with HOOK1, HOOK2 and HOOK3. Ref.7
	Sequence conflict	212	1	K → N in AAH95401. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3DD4E32980463324	FASTA	292	33,128
	10 20 30 40 50 60 MNPFWSMSTS SVRKRSEGEE KTLTGDVKTS PPRTAPKKQL PSIPKNALPI TKPTSPAPAA 70 80 90 100 110 120 QSTNGTHASY GPFYLEYSLL AEFTLVVKQK LPGVYVQPSY RSALMWFGVI FIRHGLYQDG 130 140 150 160 170 180 VFKFTVYIPD NYPDGDCPRL VFDIPVFHPL VDPTSGELDV KRAFAKWRRN HNHIWQVLMY 190 200 210 220 230 240 ARRVFYKIDT ASPLNPEAAV LYEKDIQLFK SKVVDSVKVC TARLFDQPKI EDPYAISFSP 250 260 270 280 290 WNPSVHDEAR EKMLTQKKPE EQHNKSVHVA GLSWVKPGSV QPFSKEEKTV AT
	Isoform 2. Checksum: CE781BABE3F9695C	FASTA	293	33,256
	10 20 30 40 50 60 MNPFWSMSTS SVRKRSEGEE KTLTGDVKTS PPRTAPKKQL PSIPKNALPI TKPTSPAPAA 70 80 90 100 110 120 QSTNGTHASY GPFYLEYSLL AEFTLVVKQK LPGVYVQPSY RSALMWFGVI FIRHGLYQDG 130 140 150 160 170 180 VFKFTVYIPD NYPDGDCPRL VFDIPVFHPL VDPTSGELDV KRAFAKWRRN HNHIWQVLMY 190 200 210 220 230 240 ARRVFYKIDT ASPLNPEAAV LYEKDIQLFK SKVVDSVKVC TARLFDQPKI EDPYAISFSP 250 260 270 280 290 WNPSVHDEAR EKMLTQKKKP EEQHNKSVHV AGLSWVKPGS VQPFSKEEKT VAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Dermoid cancer.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Skin.
[6]	"Regulation of apoptosis by the Ft1 protein, a new modulator of protein kinase B/Akt." Remy I., Michnick S.W. Mol. Cell. Biol. 24:1493-1504(2004) [PubMed: 14749367] [Abstract] Cited for: FUNCTION, INTERACTION WITH AKT1, SUBCELLULAR LOCATION.
[7]	"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex." Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W. Mol. Biol. Cell 19:5059-5071(2008) [PubMed: 18799622] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, ASSOCIATION WITH THE HOPS COMPLEX, MUTAGENESIS OF 106-TRP-PHE-107.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK023320 mRNA. Translation: BAB14524.1. CR457308 mRNA. Translation: CAG33589.1. AK222743 mRNA. Translation: BAD96463.1. CH471092 Genomic DNA. Translation: EAW82805.1. BC001134 mRNA. Translation: AAH01134.1. BC095401 mRNA. Translation: AAH95401.1.
IPI	IPI00008304. IPI00936141.
RefSeq	NP_001012398.1. NP_071921.1.
UniGene	Hs.380897
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H8T0. 26 interactions.
STRING	Q9H8T0.
Proteomic databases
PRIDE	Q9H8T0.
Genome annotation databases
Ensembl	ENST00000300245; ENSP00000300245; ENSG00000166971; Homo sapiens. [Genome view] ENST00000394657; ENSP00000378152; ENSG00000166971; Homo sapiens. [Genome view] ENST00000425875; ENSP00000409567; ENSG00000166971; Homo sapiens. [Genome view] ENST00000453503; ENSP00000409774; ENSG00000166971; Homo sapiens. [Genome view]
GeneID	64400.
KEGG	hsa:64400.
UCSC	uc002ehk.1. human. uc002ehm.2. human.
Organism-specific databases
CTD	64400.
GeneCards	GC16M052082.
H-InvDB	HIX0013035.
HGNC	HGNC:16710. AKTIP.
MIM	608483. gene.
PharmGKB	PA28416.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9H8T0.
OMA	VRKRSDG.
Gene expression databases
ArrayExpress	Q9H8T0.
Bgee	Q9H8T0.
CleanEx	HS_AKTIP.
Genevestigator	Q9H8T0.
GermOnline	ENSG00000166971. Homo sapiens.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	66346.
SOURCE	Search...

Entry information

Entry name

AKTIP_HUMAN

Accession

Primary (citable) accession number: Q9H8T0
Secondary accession number(s): Q503B1, Q53H38

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.