Reviewed, UniProtKB/Swiss-Prot Q97SA9 (LACG1_STRPN)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase 1
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase 1
      Short name=PGALase 1
    P-beta-Gal 1
      Short name=PBG 1

Gene names

Name:	lacG1
Synonyms:	lacG-1
Ordered Locus Names:	SP_0477

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	6-phospho-beta-galactosidase 1 HAMAP MF_01574		PRO_0000260730
Sites
	Active site	164	1	Proton donor By similarity
	Active site	378	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97SA9-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 964D7022454360C6	FASTA	470	54,633
	10 20 30 40 50 60 MENLQVKALP KEFLLGTATA AYQVEGATRV DGKGINMWDV YLQENSPFLP DPASDFYYRY 70 80 90 100 110 120 EEDIALAAEH GLQALRLSIS WVRIFPDIDG DANVLAVHYY HRVFQSCLKH NVIPFVSLHH 130 140 150 160 170 180 FDSPQKMLET GDWLNRENID RFIRYARFCF QEFTEVKHWF TINELMSLAA GQYIGGQFPP 190 200 210 220 230 240 NHHFQLSEAI QANHNMLLAH ALAVLEFHQL GIEGKVGCIH ALKPGYPIDG QKENILAAKR 250 260 270 280 290 300 YDVYNNKFLL DGTFLGYYSE DTLFHLNQIL EANNSSFIIE DGDLEIMKRA APLNTMFGMN 310 320 330 340 350 360 YYRSEFIREY KGENRQEFNS TGIKGQSSFK LNALGEFVKK PGIPTTDWDW NIYPQGLFDM 370 380 390 400 410 420 LLRIKEEYPQ HPVIYLTENG TALKEVKPEG ENDIIDDSKR IRYIEQHLHK VLEARDRGVN 430 440 450 460 470 IQGYFIWSLQ DQFSWANGYN KRYGLFFVDY ETQKRYIKKS ALWVKGLKRN

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005672 Genomic DNA. Translation: AAK74636.1.
PIR	C95055.
RefSeq	NP_344996.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	930415.
GenomeReviews	Gene locus SP_0477 in contig AE005672_GR.
KEGG	spn:SP_0477.
TIGR	SP_0477.
Phylogenomic databases
HOGENOM	Q97SA9.
OMA	FSIAWGR.
Enzyme and pathway databases
BRENDA	3.2.1.85. 600.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG1_STRPN

Accession

Primary (citable) accession number: Q97SA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)