Reviewed, UniProtKB/Swiss-Prot Q8YJT1 (DCUP_BRUME)

Names and origin

Protein names

Recommended name:
    Uroporphyrinogen decarboxylase
      Short name=URO-D
      Short name=UPD
    EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	BMEI0001

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity.
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP MF_00218
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Uroporphyrinogen decarboxylase HAMAP MF_00218		PRO_0000187589
Regions
	Region	23 – 27	5	Substrate binding By similarity
Sites
	Binding site	42	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Site	73	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJT1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 383CA94E1010D3F9	FASTA	341	37,788
	10 20 30 40 50 60 MNLKVLKVID GETVFPPPIW MMRQAGRYLP EYRETRKKAG SFLDLCYSPD LAVEVTLQPI 70 80 90 100 110 120 RRFGFDAAIL FSDILVVPHA LGRDLRFEEG KGPLMTPIDA DEIFWLETEG VAKRLEPVYE 130 140 150 160 170 180 TVRLVREQLP DETTLLGFCG APWTVATYMI AGHGTPDQAP ARLFAYRFPE AFEKLLNDLA 190 200 210 220 230 240 DVSAEYLIEQ LGAGADAVQI FDSWSGVLDE DCFERFCIRP VARIVQKVRA VYPQARIIGF 250 260 270 280 290 300 PKGAGMLYAG YREKTGVDML GLDWSVPLSF AALLQEEGAV QGNLDPLRVV AGGNALDEGV 310 320 330 340 DAILERMGQG PLVFNLGHGI TPQAPIENVQ RMIDRVRGGK S

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51183.1.
PIR	AD3252.
RefSeq	NP_538919.1.
3D structure databases
HSSP	HSSP built from PDB template 1JPH based on UniProtKB P06132.
ModBase	Search...
Genome annotation databases
GeneID	1195727.
GenomeReviews	Gene locus BMEI0001 in contig AE008917_GR.
KEGG	bme:BMEI0001.
NMPDR	fig|224914.1.peg.1.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJT1.
OMA	VFTKGGG.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0001-MON.
BRENDA	4.1.1.37. 277959.
Family and domain databases
HAMAP	MF_00218. [Tree]
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
PANTHER	PTHR21091:SF2. HemE. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
ProDom	PD003225. Uro_decarbxyls. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01464. hemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_BRUME

Accession

Primary (citable) accession number: Q8YJT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)