Reviewed, UniProtKB/Swiss-Prot Q8G5W9 (SYA_BIFLO)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BL0882

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075066

Sequences

	Sequence		Length	Mass (Da)
	Q8G5W9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D10D2CF8AF4AA0D5	FASTA	893	97,444
	10 20 30 40 50 60 MRTADIAKRY LDYFAKHDHL IVPSASLISP NPTTLFTIAG MVPFIPYLMG EQTPPKRRMA 70 80 90 100 110 120 SNQKCVRTLD IDEVGKTTRH GTFFQMLGNF SFGDYFKEEA IHYAWELLTT SQDEGGYGFD 130 140 150 160 170 180 PEKLWMTTFT DDDEARSMWI NEGVDPEHIQ KMGMEDNFWT TGGPGPGGPC SEIYVDRGPA 190 200 210 220 230 240 FGKEGGPIAD ENRYIEIWDL VFENYEVDNV KSKTDLHIVG ELENKNIDTG AGLERLAYLL 250 260 270 280 290 300 QGKNNIYETD EVFPVIEAAE QLSGLKYGEN EDADVRFRVV ADHVRSALMI MSDGVRPSNV 310 320 330 340 350 360 GRGYVLRRLL RRTVRSMRML GVTDPVLPTL FPTSKAAMEA SYPELNDTFH EVSESAYGEE 370 380 390 400 410 420 DAFRRTLETG TTILDVAVNK AKSDSAEPVV AGEDAFKLHD TYGFPIELTL EMAAEQGVKV 430 440 450 460 470 480 DEAKFRELMA EQKSRARADA LKKRHNVDLS VYDDFKKTLV SPIDFLGYTD MSARAKVIGI 490 500 510 520 530 540 MQEGKGSVPA VTGPANVEVI LDRTPFYAEA GGQLADQGEI LSDDGAVLEV DDVQKPIKDL 550 560 570 580 590 600 IVHQCRLTEG TLVVGAEVNA NIDLARRGAI ARSHTATHMV HKALREELGP QATQRGSEDA 610 620 630 640 650 660 PNRLRFDFQW SKAPAKSVIS AVEERVNDKL RDNLAVTTKE MKFDDAIALG AMHLFGEKYG 670 680 690 700 710 720 DIVRVVSIGE DGWSRELCGG THVDHVGKIG MVNILSEASI GSGVRRVDAV VGQGAYDFNA 730 740 750 760 770 780 REHALVSQLS DKLNARPDEL AERVNALLAK LKESDRRLAS MYESQLAASV PALVADTKNS 790 800 810 820 830 840 AAPVKVAVKN VGHFGAVDAL RKTVLDVRAQ LGEDAPVVVA LAGVNEDDKP MVAVATNEAA 850 860 870 880 890 RKAGIKAGDL VRGAAKVLGG GGGGKPDFAQ GGGVDASKID EALEALKHEA QKA

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24695.1.
RefSeq	NP_696059.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1022383.
GenomeReviews	Gene locus BL0882 in contig AE014295_GR.
KEGG	blo:BL0882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8G5W9.
OMA	TLMFTNS.
Enzyme and pathway databases
BioCyc	BLON206672:BL0882-MON.
BRENDA	6.1.1.7. 39917.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BIFLO

Accession

Primary (citable) accession number: Q8G5W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)