Reviewed, UniProtKB/Swiss-Prot Q60132 (T2C9_CITFR)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Cfr9I
      Short name=R.Cfr9I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Cfr9I
    Endonuclease Cfr9I

Gene names

Name:

cfr9IR

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCCGGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Type-2 restriction enzyme Cfr9I		PRO_0000077294

Sequences

	Sequence		Length	Mass (Da)
	Q60132-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6DD85FF9DA98117E	FASTA	330	36,806
	10 20 30 40 50 60 MTNKIVFPEP KQQVDFAFSL KRFRGIYLQN ALLETVRDMD IVALDTQLAE YVNKADLATL 70 80 90 100 110 120 ATYGLRAELL FPVPVLLETN PFLLGYYRLL MGYSQKEFYG KDKGFNAGCF KSMEVKGNIG 130 140 150 160 170 180 KVAKPKISEL CHAFCSVASS LLQGVGPLRI SRELLDDLTL LTVGPQLRGG ANNQRGADGI 190 200 210 220 230 240 VLVFEIIKEI VSHAVAEVRE NAIEVNSATG RNVLIEFAPD PDIIIREEMS LDNYRNVVAI 250 260 270 280 290 300 EVKSGTDVSN IHNRIGEAEK SHQKARGHGY TECWTVVNVS RLDMDKARKE SPSTNRFYSI 310 320 330 TDLSLREGEQ YEDFRRRVLS LTAISAAPTP

References

[1]	"Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system." Lubys A., Menkevicius S., Timinskas A., Butkus V., Janulaitis A. Gene 141:85-89(1994) [PubMed: 8163180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL9.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17022 Genomic DNA. Translation: CAA34888.1. X74517 Genomic DNA. Translation: CAA52628.1.
PIR	I40700.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	699. Cfr9I.
Enzyme and pathway databases
BRENDA	3.1.21.4. 2347.
Family and domain databases
InterPro	IPR019071. Restrict_endonuc_II_XcyI. [Graphical view]
Pfam	PF09571. RE_XcyI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2C9_CITFR

Accession

Primary (citable) accession number: Q60132

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)