Reviewed, UniProtKB/Swiss-Prot Q5PBJ0 (SYA_ANAMM)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AM224

Organism

Anaplasma marginale (strain St. Maries) [Complete proteome] [HAMAP]

Taxonomic identifier

234826 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075046

Sequences

	Sequence		Length	Mass (Da)
	Q5PBJ0-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 62FCE6D20CB03F1F	FASTA	882	95,968
	10 20 30 40 50 60 MSSGSLSSIR KKFLEFFVKN GHKLYASAPL VATGDASLLF TSAGMVPFKQ AFTSGSSVDG 70 80 90 100 110 120 AKTAVSSQKC LRAGGKHNDL ENVGYTNRHH TFFEMLGNFS FGDYFKERAI ELAWRFVTEE 130 140 150 160 170 180 MCLSKDRLWI TVYSEDQEAF DIWKKITGFP DDRIIKISTS DNFWSMGDTG PCGPCSEIFY 190 200 210 220 230 240 DYGEHVQGGP PGSKDADGPR FTEVWNLVFM QFCRDEHGEL NPLPHKCIDT GMGLERAAAV 250 260 270 280 290 300 VQGVCDNYDT DLFKAVIRKS QDVFGSPDNS IAHRVIADHI RAAAFLISEG LSPGNEGRNY 310 320 330 340 350 360 VLRRIIRRAV RYAYQLDPSN VAIHEVLPVL TKEGSAGYMG DAYPELVRCE QSITSTLRSE 370 380 390 400 410 420 GEGFVDTLRR GMALLEKEIG GLSPGQVLLG DVAFKLYDTF GFPLDITLDI AKERGLKFDQ 430 440 450 460 470 480 EGFNKGMGEQ KARSRKHWVG SGEDASHRLW EELQAQHKNT RFVGYDCCST KASVLSITRD 490 500 510 520 530 540 GMAVQSVNSG EKACLLLDVS PFYAESGGQE GDKGSITGVS GVKNSGGANI AEVTYTRKAS 550 560 570 580 590 600 NLHIHECTIT SGVFNVGDTV DAAIDVDRRE RLKANHSATH ILHSVLRTHI DGNIQQKGSL 610 620 630 640 650 660 VAEDKLRFDF NYASALTKEQ IALIEREVNR RIMSNKPVLT DHCSFEAAVQ GGAIALFGEK 670 680 690 700 710 720 YSEHSVRVVS MGDSKELCGG THVRYTGDIG AFKIVSESGI ALGVRRIEAI TGQSVVDGLR 730 740 750 760 770 780 KDGDILLHIS ERLGVPVGEV SEGLERLLKE KLELKKKLVC AWHEIIKSSI SPVNCGAGVV 790 800 810 820 830 840 LHCGYFPTIP VDAIMEFMKS ARKAERGIFA IATAVDGKAV LIIGVGDTAS KVLGASELVK 850 860 870 880 TLADLQGKGG GNAGLARVSL EVGNVQEALS TILNKVTAAF PT

References

[1]

"Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins." Brayton K.A., Kappmeyer L.S., Herndon D.R., Dark M.J., Tibbals D.L., Palmer G.H., McGuire T.C., Knowles D.P. Jr. Proc. Natl. Acad. Sci. U.S.A. 102:844-849(2005) [PubMed: 15618402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000030 Genomic DNA. Translation: AAV86339.1.
RefSeq	YP_153594.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5PBJ0.
Genome annotation databases
GeneID	3171157.
GenomeReviews	Gene locus AM224 in contig CP000030_GR.
KEGG	ama:AM224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PBJ0.
OMA	TLMFTNS.
Enzyme and pathway databases
BioCyc	AMAR234826:AM224-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ANAMM

Accession

Primary (citable) accession number: Q5PBJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)