Reviewed, UniProtKB/Swiss-Prot Q3MGN4 (SYA_ANAVT)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Ava_0226

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347487

Sequences

	Sequence		Length	Mass (Da)
	Q3MGN4-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 1BCFE443E9011EEA	FASTA	880	96,994
	10 20 30 40 50 60 MSSNPQHLSG NEIRTRFLDF YAQRGHQILA SASLVPEDPT VLLTIAGMLP FKPIFLGQRT 70 80 90 100 110 120 PEFKRATTSQ KCIRTNDIEN VGRTKRHQTF FEMLGNFSFG DYFKEQAIAW GWEISTEVFG 130 140 150 160 170 180 LPKERLVVSV FEEDDEAYVI WRDQIGVTEA RIKRMGADDN FWVSGPTGPC GPCSEIYYDF 190 200 210 220 230 240 HPERGDENID LEDDTRFIEF YNLVFMQYNR DASGNLTPLQ NKNIDTGMGL ERITQILQQV 250 260 270 280 290 300 PNNYETDLIF PIIETAAKIA GIDYHSSDET TKVSLKVIGD HVRSVVHMIA DEIRASNVGR 310 320 330 340 350 360 GYVLRRLIRR VVRHGRLIGI SGEFINQVAE RAIALSESAY PNVRKRETVI KAELEREEAN 370 380 390 400 410 420 FLKTLDRGEK LLEEIIQQVK KQGQTIISGE SAFTLYDTYG FPLELTQEIA EENNLTVDED 430 440 450 460 470 480 DFNVEMQKQV ERAKAAHETI DLTVQGSLDK LAEHIHATEF IGYSQATATA KVEVLLVDGV 490 500 510 520 530 540 VQEEAEAGTE VQIVLDKTPF YAESGGQIGD RGYISGDGIV VQVEDVKKES DFFVHFGRIE 550 560 570 580 590 600 RGTLRVGDSV TAQIDRAGRR RAQANHTATH LLQAALKTIV DGGISQAGSL VSFDRLRFDF 610 620 630 640 650 660 NSPRGLTAEE IQQVEEQINT WIAEAHSAKI EVLPLAEAKA RGAVAMFGEK YGDEVRVIDF 670 680 690 700 710 720 PGVSMELCGG THVSNTAEIG VFKIISEAGV ASGVRRIEAV SGLAVLDYLN VRDKVVKDLS 730 740 750 760 770 780 DRFKVKPEEL PERITTLQNE LRTTEKQLET LKGQLAIAKS DSLLQTADTL GDHKIIVAQL 790 800 810 820 830 840 EGVDPESLKS AAERLLQKIG NGAVVLGSVP EADKVSLVAA FSPEVNKKGL QAGKFIGAIA 850 860 870 880 KICGGGGGGR PNLAQAGGRD ASKLPTALEQ AQSELKSALG

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA19852.1.
RefSeq	YP_320747.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3MGN4.
Genome annotation databases
GeneID	3683029.
GenomeReviews	Gene locus Ava_0226 in contig CP000117_GR.
KEGG	ava:Ava_0226.
NMPDR	fig|240292.3.peg.546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3MGN4.
OMA	GEFVIKL.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_0226-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ANAVT

Accession

Primary (citable) accession number: Q3MGN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)