Reviewed, UniProtKB/Swiss-Prot Q1IJG7 (SYA_ACIBL)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Acid345_3983

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	1098 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1098	1098	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347469

Sequences

	Sequence		Length	Mass (Da)
	Q1IJG7-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: AD8FFAAA5C9A1D5A	FASTA	1,098	120,722
	10 20 30 40 50 60 MTGRCLIKYS FTPMMTGSQV RRRFLDFFIS KGHKEVHSSS LVPANDPTLL FTNAGMNQFK 70 80 90 100 110 120 DVFLGVEKRD YNRATTSQKC VRAGGKHNDL ENVGFTNRHH TFFEMLGNFS FGDYFKKDAI 130 140 150 160 170 180 AYAWELVTSP DWYGMQQDKL YATIFKGENG VPRDDEAYQL WLDVGVPKER VFEMGMKDNF 190 200 210 220 230 240 WAMGDTGPCG PCSELHYDMG VASSDKKNPE CAAGECKFPC ECGRYVEIWN LVFMQFDRDA 250 260 270 280 290 300 SGTLNPLPKP SIDTGAGLER VTAVMQGVVS NYDTDLFTPL IKRAAELTDV DEKREEAKES 310 320 330 340 350 360 QSKSAASLRV IADHSRAATF LISDGVIPAN EGRGYVLRKI IRRGIRHGRL LGQNKPFLHD 370 380 390 400 410 420 MVYAVRDLMQ DAYPELKETA DRVAKTVLAE ETRFANTLDI GLKKLDEDLA SLAETTRELG 430 440 450 460 470 480 RLSWEEPKPV EFALKEVARH VFVVAQMLSP GMTFDTPGHG FLLRRGIYRA WMDLKAVGLD 490 500 510 520 530 540 RVVRLSEIAE ALSEGETGRV QSVAKNLAHT KQILDAEQER LQSASSQLEF ELDAMEAEKK 550 560 570 580 590 600 LAENPEMLAE QVREVFGPAL EEKVINELRG KLQSRPVYSG DKAFKLYDTF GLPLDFMVDA 610 620 630 640 650 660 ARDQGIEFDQ AGFDAAMESQ RETARASWKG GSKLSASPIY RELAEELGTK PYPGQLAGGH 670 680 690 700 710 720 PMITPQSIFV GYTSTEFKGA TVLAIISNGA SVAQLAPGDV AEMVLDYTPF YSESGGQIGD 730 740 750 760 770 780 TGWLYDSTGN TVVAEVETVQ SPVQGVRAHK VTARQNIAVG DKLNAVVNAD VRRATMRNHT 790 800 810 820 830 840 GTHLLHAALR EVLGKHVKQA GSLVDPAKLR FDFSHFTGVA DEELQDIEDI VNKEVLKNDR 850 860 870 880 890 900 VEVIENVPID VAVNEYKAMA LFGEKYGDRV RVIKIGDFST ELCGGTHTLA TGEIGLIKVL 910 920 930 940 950 960 HEGSVSSGVR RLEAVTGENS VRHFRKDHEL EGVVSTIVRP SEGLSPAQAL KFELDRREEE 970 980 990 1000 1010 1020 IKKLRKELEQ SRMKSASSAV SSATESAREV KGIKVLATRA DNIDRNQMRT LIDNLRSKLG 1030 1040 1050 1060 1070 1080 SGVIVLGSVQ DGKVALIVGV TKDLTSKIQA GKIIAQVAKH VGGSGGGRPD MAEAGGKDPA 1090 ALDGALNATY GIVDSLLS

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000360 Genomic DNA. Translation: ABF42983.1.
RefSeq	YP_593057.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IJG7.
Genome annotation databases
GeneID	4072456.
GenomeReviews	Gene locus Acid345_3983 in contig CP000360_GR.
KEGG	aba:Acid345_3983.
NMPDR	fig|204669.6.peg.3967.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IJG7.
OMA	GEFVIKL.
Family and domain databases
HAMAP	MF_00036. Divergent sequence. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 3 hits. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACIBL

Accession

Primary (citable) accession number: Q1IJG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 13, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)