Reviewed, UniProtKB/Swiss-Prot P97265 (GBA3_CAVPO)

Names and origin

Protein names

Recommended name:
    Cytosolic beta-glucosidase
    EC=3.2.1.21

Gene names

Name:	Gba3
Synonyms:	Cbg

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Enzyme regulation	Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside. Ref.2
Subcellular location	Cytoplasm › cytosol. Ref.2
Tissue specificity	Present in hepatocytes (at protein level). Ref.2
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cytosolic beta-glucosidase		PRO_0000063907
Sites
	Active site	165	1	Proton donor Potential
	Active site	373	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P97265-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 9ED6B5BA89C22EE6	FASTA	469	53,745
	10 20 30 40 50 60 MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD VACGSYTLWE 70 80 90 100 110 120 EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY NKIIDDLLTN GVTPVVTLYH 130 140 150 160 170 180 FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF STFGNRVRQW ITINEPNVLC AMGYDLGFFA 190 200 210 220 230 240 PGVSQIGTGG YQAAHNMIKA HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ 250 260 270 280 290 300 KAAERAINFQ FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT 310 320 330 340 350 360 ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP WGIRKLLNYI 370 380 390 400 410 420 KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL FKAIHVDKVN LQLYCAWSLL 430 440 450 460 DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY TSAKEYAKII RNNGLERPQ

References

[1]

"Primary structure of the cytosolic beta-glucosidase of guinea pig liver." Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H. Biochem. J. 319:829-837(1996) [PubMed: 8920987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS. Strain: Dunkin-Hartley. Tissue: Liver.

[2]

"Expression of cytosolic beta-glucosidase in guinea pig liver cells." Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E. Hepatology 28:156-163(1998) [PubMed: 9657108] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U50545 mRNA. Translation: AAB41058.1.
3D structure databases
HSSP	HSSP built from PDB template 1OIF based on UniProtKB Q08638.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
Ensembl	ENSCPOT00000019748; ENSCPOP00000014554; ENSCPOG00000024273; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	P97265.
Enzyme and pathway databases
BRENDA	3.2.1.21. 44.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
ProDom	PD000650. Glyco_hydro_euk. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GBA3_CAVPO

Accession

Primary (citable) accession number: P97265

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	May 1, 1997
Last modified:	July 28, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)