Reviewed, UniProtKB/Swiss-Prot P94147 (MTA1_RUEGE)

Names and origin

Protein names

Recommended name:
    Modification methylase AgeI
      Short name=M.AgeI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase AgeI

Gene names

Name:

ageIM

Organism

Ruegeria gelatinovora (Agrobacterium gelatinovorum)

Taxonomic identifier

53501 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Thalassobius

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Modification methylase AgeI		PRO_0000087856
Sites
	Active site	80	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P94147-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 97C5EC5414B02FD3	FASTA	429	47,359
	10 20 30 40 50 60 MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD SIETVDPKKI 70 80 90 100 110 120 REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV PYFGFVEEFR PKAFLIENVV 130 140 150 160 170 180 GLLSMSGGAV LADMVARAEA LGYAADVVTL DACEYGVPQH RRRVFIFGAA DGQRIDPPQP 190 200 210 220 230 240 SHVNGKRSGV VLNDQPSLFF DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT 250 260 270 280 290 300 EYQRLMRGNS TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG 310 320 330 340 350 360 GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR LRWDAPSHTV 370 380 390 400 410 420 VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS QFRQFRQIGN AVPPLLGRAM AETIKVAIS

References

[1]	"Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium." Suzuki T., Sugimoto E., Tahara Y., Yamada Y. Biosci. Biotechnol. Biochem. 60:444-447(1996) [PubMed: 8901102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 12617.
[2]	"Method for cloning and producing AgeI restriction endonuclease in E.coli." Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R. Patent number EP0959131, 26-APR-1999 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 12617.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78259 Genomic DNA. Translation: BAA11333.1. AF247972 Genomic DNA. Translation: AAF71525.1.
PIR	JC4656.
3D structure databases
HSSP	HSSP built from PDB template 1DCT based on UniProtKB P20589.
ModBase	Search...
Protein family/group databases
REBASE	3276. M.AgeI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTA1_RUEGE

Accession

Primary (citable) accession number: P94147

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 1997
Last modified:	March 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)