Reviewed, UniProtKB/Swiss-Prot P52558 (PUR6_BRUME)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase catalytic subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purE
Ordered Locus Names:	BMEI0296

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Phosphoribosylaminoimidazole carboxylase catalytic subunit		PRO_0000074970
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	41	1	Substrate By similarity
Experimental info
	Sequence conflict	55	1	A → R in AAA57002. Ref.1
	Sequence conflict	67 – 68	2	GA → R in AAA57002. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52558-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 0B812230C4EE9EE7	FASTA	162	16,596
	10 20 30 40 50 60 MSVDVAIIMG SQSDWETMHH AADTLEALGI SFDARIVSAH RTPDRLVAFA KGAKAEGFKV 70 80 90 100 110 120 IIAGAGGAAH LPGMAAAMTP LPVFGVPVQS KALSGQDSLL SIVQMPAGIP VGTLAIGRAG 130 140 150 160 AVNAALLAAA VLALYDEALA ARLDEWRKAQ TESVAERPSN EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and genetic characterization of the purEK operon of Brucella melitensis strain 16M." Warren R., Hoover D., Hadfield T., Drazek S. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10241 Unassigned DNA. Translation: AAA57002.1. AE008917 Genomic DNA. Translation: AAL51477.1. Different initiation.
PIR	AB3289.
RefSeq	NP_539213.1.
3D structure databases
HSSP	HSSP built from PDB template 1QCZ based on UniProtKB P09028.
ModBase	Search...
Genome annotation databases
GeneID	1196007.
GenomeReviews	Gene locus BMEI0296 in contig AE008917_GR.
KEGG	bme:BMEI0296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P52558.
OMA	GVVMGSS.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0296-MON.
BRENDA	4.1.1.21. 277959.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
PANTHER	PTHR23046. AIR_carboxyl. 1 hit.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR6_BRUME

Accession

Primary (citable) accession number: P52558

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2002
Last modified:	November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)