Reviewed, UniProtKB/Swiss-Prot P50475 (SYAC_RAT)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:

Aars

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subunit structure	Monomer.
Subcellular location	Cytoplasm Potential.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs Potential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Ref.2 Inferred from direct assay. Source: RGD
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: RGD
Molecular function	ATP binding Ref.2 Inferred from physical interaction. Source: RGD alanine-tRNA ligase activity Ref.2 Inferred from direct assay. Source: RGD amino acid binding Ref.2 Inferred from physical interaction. Source: RGD tRNA binding Ref.2 Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 968	968	Alanyl-tRNA synthetase, cytoplasmic		PRO_0000075284
Regions
	Region	1 – 497	497	Catalytic By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	19	1	N6-acetyllysine By similarity
	Modified residue	555	1	Phosphoserine By similarity
	Modified residue	578	1	Phosphothreonine By similarity
	Modified residue	580	1	Phosphotyrosine By similarity
	Modified residue	876	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	459	1	G → E AA sequence Ref.2
	Sequence conflict	474 – 476	3	RAK → ARA AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50475-1 [UniParc]. Last modified October 13, 2009. Version 3. Checksum: 10BF81F46F7AF546	FASTA	968	106,790
	10 20 30 40 50 60 MDSTLTAREI RERFINYFKR NEHTYVHSSA TIPLDDPTLL FANAGMNQFK PIFLNTVDPS 70 80 90 100 110 120 HPMAKLSRAA NTQKCIRAGG KHNDLDDVGK DVYHHTFFEM LGSWSFGDYF KELACKMALE 130 140 150 160 170 180 LLTQEFGIPV ERLYVTYFGG DEAAGLEPDL ECRQIWQNLG LDEAKILPGN MKDNFWEMGD 190 200 210 220 230 240 TGPCGPCSEI HYDRIGGRDA AHLVNQDDPN VLEIWNLVFI QYNRESDGVL KPLPKKSIDT 250 260 270 280 290 300 GMGLERLVSV LQNKMSNYDT DLFVPYFEAI QKGTGARPYT GKVGAEDTDG IDMAYRVLAD 310 320 330 340 350 360 HARTITVALA DGGRPDNTGR GYVLRRILRR AVRYSHEKLN ASRGFFATLV DVVVQSLGDA 370 380 390 400 410 420 FPELKKDPDM VKDIINEEEV QFLKTLSRGR RILDRKIQSL GDCQTIPGDT AWLLYDTYGF 430 440 450 460 470 480 PVDLTGLIAE EKGLVVDMDG FEEERKLAQL KSQGKGAGGE DLIMLDIYAI EELRAKGLEA 490 500 510 520 530 540 TDDSPKYNYH SDSSGSYVFE CTVATVLALR REKMFVDEVV TGQECGVVLD KTCFYAEQGG 550 560 570 580 590 600 QIFDEGYLVK VDDSSEDKTE FTVKNAQVRG GYVLHIGTIY GNLRVGDQVR LFIDEPRRRP 610 620 630 640 650 660 VMSNHTATHI LNFALRSVLG DADQKGSLVA PDRLRFDFTA KGAMSTEQIK KTEEIVNGMI 670 680 690 700 710 720 EAAKPVYTLD CPLAAAKAIQ GLRAVFDETY PDPVRVVSIG VPVSELLDDP SGPAGSLTSV 730 740 750 760 770 780 EFCGGTHLRN SSHAGAFVIV TEEAIAKGIR RIVAVTGAEA QKALRKSETL KKSLSAMEVK 790 800 810 820 830 840 VKAQSAPNKD VQKEIADLGE VLATAVIPQW QKDEQRETLK SLKKVMDDLD RASKADVQKR 850 860 870 880 890 900 VLEKTKQLID SNPNQPLVIL EMESGASAKA LNEALKLFKT HSPQTSAMLF TVDNEAGKIT 910 920 930 940 950 960 CLCQVPQNAA NRGLKASEWV QQVSGLMDGK GGGKDMSAQA TGKNVGCLQE ALQLATSFAQ LRLGDVKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Alanyl-tRNA synthetase from Escherichia coli, Bombyx mori and Ratus ratus. Existence of common structural features." Dignam J.D., Dignam S.S., Brumley L.L. Eur. J. Biochem. 198:201-210(1991) [PubMed: 2040280] [Abstract] Cited for: PROTEIN SEQUENCE OF 456-476. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 600-968. Tissue: Thymus.
[4]	Maurya D.K., Bhargava P. Submitted (JAN-2009) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH473972 Genomic DNA. Translation: EDL92558.1. BC098738 mRNA. Translation: AAH98738.1.
IPI	IPI00363563.
PIR	S16073.
RefSeq	NP_001093987.1.
UniGene	Rn.8645
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	292023.
Organism-specific databases
RGD	1304832. Aars.
Phylogenomic databases
HOVERGEN	P50475.
Enzyme and pathway databases
BRENDA	6.1.1.7. 248.
Gene expression databases
Genevestigator	P50475.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYAC_RAT

Accession

Primary (citable) accession number: P50475
Secondary accession number(s): A6IZ80, Q4G057

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 13, 2009
Last modified:	November 3, 2009
This is version 50 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)