Reviewed, UniProtKB/Swiss-Prot P38972 (PUR4_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	ADE6
Ordered Locus Names:	YGR061C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 40800 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct phosphoribosylformylglycinamidine synthase activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-14246,EBI-14246
VAC14	Q06708	2	EBI-14246,EBI-27189

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1358	1358	Phosphoribosylformylglycinamidine synthase		PRO_0000100405
Regions
	Domain	1093 – 1358	266	Glutamine amidotransferase type-1
	Nucleotide binding	345 – 356	12	ATP Potential
Sites
	Active site	1187	1	For GATase activity By similarity
Amino acid modifications
	Modified residue	333	1	Phosphothreonine Ref.4
Experimental info
	Sequence conflict	84	1	A → L in AAA50357. Ref.1
	Sequence conflict	219 – 220	2	DS → EC in AAA50357. Ref.1
	Sequence conflict	226	1	L → V in AAA50357. Ref.1
	Sequence conflict	492	1	A → P in AAA50357. Ref.1
	Sequence conflict	499	1	G → R in AAA50357. Ref.1
	Sequence conflict	551	1	A → T in AAA50357. Ref.1
	Sequence conflict	604	1	E → K in AAA50357. Ref.1
	Sequence conflict	1169 – 1170	2	SQ → TSSK in AAA50357. Ref.1
	Sequence conflict	1298	1	G → A in AAA50357. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38972-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: A48A24ECB1BE7973	FASTA	1,358	148,905
	10 20 30 40 50 60 MTDYILPGPK ALSQFRVDNL IKDINSYTNS TSVINELRSC YIHYVNGIAQ NLSEQDTKLL 70 80 90 100 110 120 EVLLTYDSAL DIANDPLARQ LNDAVANNLP SSALGEDTYL IRVVPRSGTI SPWSSKATNI 130 140 150 160 170 180 AHVCGLQDKV QRIERGLALL IKTVPGFPLL ENLNDISLKC VYDRMTQQLY LTEPPNTMSI 190 200 210 220 230 240 FTHEEPKPLV HVPLTPKDTK QSPKDILSKA NTELGLALDS GEMEYLIHAF VETMKRDPTD 250 260 270 280 290 300 VELFMFAQVN SEHCRHKIFN ADWTIDGIKQ QFTLFQMIRN THKLNPEYTI SAYSDNAAVL 310 320 330 340 350 360 DSENDAFFFA PNSTTKEWTS TKERIPLLIK VETHNHPTAV SPFPGAATGS GGEIRDEGAT 370 380 390 400 410 420 GRGSKTKCGL SGFSVSDLLI PGNEQPWELN IGKPYHIASA LDIMIEAPLG SAAFNNEFGR 430 440 450 460 470 480 PCINGYFRTL TTKVLNHQGK EEIRGFHKPI MIAGGFGTVR PQFALKNTPI TPGSCLIVLG 490 500 510 520 530 540 GQSMLIGLGG GAASSVASGE GSADLDFASV QRGNPEMERR CQQVIDACVA LGNNNPIQSI 550 560 570 580 590 600 HDVGAGGLSN ALPELVHDND LGAKFDIRKV LSLEPGMSPM EIWCNESQER YVLGVSPQDL 610 620 630 640 650 660 SIFEEICKRE RAPFAVVGHA TAEQKLIVED PLLKTTPIDL EMPILFGKPP KMSRETITEA 670 680 690 700 710 720 LNLPEANLSE IPSLQDAIQR VLNLPSVGSK SFLITIGDRS VTGLIDRDQF VGPWQVPVAD 730 740 750 760 770 780 VGVTGTSLGE TIISTGEAMA MGEKPVNALI SASASAKLSV AESLLNIFAA DVKSLNHIKL 790 800 810 820 830 840 SANWMSPASH QGEGSKLYEA VQALGLDLCP ALGVAIPVGK DSMSMKMKWD DKEVTAPLSL 850 860 870 880 890 900 NITAFAPVFN TSKTWTPLLN RNTDDSVLVL VDLSAKQETK SLGASALLQV YNQVGNKSPT 910 920 930 940 950 960 VYDNAILKGF LESLIQLHQQ KEDIVLAYHD RSDGGLLITL LEMAFASRCG LEINIDGGDL 970 980 990 1000 1010 1020 ESQLTNLFNE ELGAVFQISA KNLSKFEKIL NENGVAKEYI SIVGKPSFQS QEIKIINSTT 1030 1040 1050 1060 1070 1080 NDVIYANSRS ELEQTWSKTS YEMQKLRDNP KTAEEEFASI TDDRDPGLQY ALTYNPADDM 1090 1100 1110 1120 1130 1140 KIGLELSSQR PKVAILREQG VNGQMEMAWC FQQAGFNSVD VTMTDLLEGR FHLDDFIGLA 1150 1160 1170 1180 1190 1200 ACGGFSYGDV LGAGAGWAKS VLYHEGVRSQ FSKFFNERQD TFAFGACNGC QFLSRLKDII 1210 1220 1230 1240 1250 1260 PGCENWPSFE RNVSEQYEAR VCMVQISQEK DNSSEESVFL NGMAGSKLPI AVAHGEGKAT 1270 1280 1290 1300 1310 1320 FSKSAEQLEK FEKDGLCCIR YVDNYGNVTE RFPFNPNGST NGIAGIKSPN GRVLAMMPHP 1330 1340 1350 ERVCRLEANS WYPEGKYEEW GGYGPWIRLF RSARRWVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae formylglycinamide ribonucleotide synthetase." Andreichuk Y.V., Domkin V.D. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U14566 Genomic DNA. Translation: AAA50357.1. Z72846 Genomic DNA. Translation: CAA97063.1.
PIR	S64356.
RefSeq	NP_011575.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1175N.
IntAct	P38972. 11 interactions.
STRING	P38972.
Proteomic databases
PeptideAtlas	P38972.
PRIDE	P38972.
Genome annotation databases
Ensembl	YGR061C; YGR061C; YGR061C; Saccharomyces cerevisiae. [Genome view]
GeneID	852952.
GenomeReviews	Gene locus YGR061C in contig Y13135_GR.
KEGG	sce:YGR061C.
NMPDR	fig|4932.3.peg.2691.
Organism-specific databases
CYGD	YGR061c.
SGD	S000003293. ADE6.
Phylogenomic databases
HOGENOM	P38972.
OMA	PHRDFLN.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-486.
BRENDA	6.3.5.3. 250.
Gene expression databases
ArrayExpress	P38972.
Genevestigator	P38972.
GermOnline	YGR061C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972711.

Entry information

Entry name

PUR4_YEAST

Accession

Primary (citable) accession number: P38972

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)