Reviewed, UniProtKB/Swiss-Prot P38632 (NSE2_YEAST)

Names and origin

Protein names

Recommended name:
    E3 SUMO-protein ligase MMS21
    EC=6.3.2.-
Alternative name(s):
    Methyl methanesulfonate-sensitivity protein 21
    Non-structural maintenance of chromosome element 2
      Short name=Non-SMC element 2

Gene names

Name:	MMS21
Synonyms:	NSE2
Ordered Locus Names:	YEL019C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Ref.6
Pathway	Protein modification; protein sumoylation.
Subunit structure	Component of the Smc5-Smc6 complex which consists of KRE29, NSE1, NSE2/MMS21, NSE3, NSE4, NSE5, SMC5 and SMC6. Ref.6
Subcellular location	Nucleus. Cytoplasm. Ref.6 Ref.4
Miscellaneous	Present with 2800 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the NSE2 family. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Ref.6 Inferred from mutant phenotype. Source: SGD modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Smc5-Smc6 complex Ref.6 Inferred from physical interaction. Source: SGD cytoplasm Ref.4 Inferred from direct assay. Source: SGD nuclear envelope Ref.6 Inferred from direct assay. Source: SGD
Molecular function	SUMO ligase activity Ref.6 Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	E3 SUMO-protein ligase MMS21		PRO_0000218991
Regions
	Zinc finger	169 – 246	78	SP-RING-type
Experimental info
	Sequence conflict	124	1	E → D in AAA20471. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38632-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: F200378289B1CEE4	FASTA	267	30,354
	10 20 30 40 50 60 MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE 70 80 90 100 110 120 EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP 130 140 150 160 170 180 KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI 190 200 210 220 230 240 ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI 250 260 MELRCKIAKM KESQEQDKRS SQAIDVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of the MMS21 gene of yeast." Williams T.L., Montelone B.A. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D7.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization." Zhao X., Blobel G. Proc. Natl. Acad. Sci. U.S.A. 102:4777-4782(2005) [PubMed: 15738391] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12311 Genomic DNA. Translation: AAA20471.1. U18530 Genomic DNA. Translation: AAB64496.1. AY558441 Genomic DNA. Translation: AAS56767.1.
PIR	S50440.
RefSeq	NP_010896.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4100N.
IntAct	P38632. 5 interactions.
STRING	P38632.
Proteomic databases
PeptideAtlas	P38632.
Genome annotation databases
Ensembl	YEL019C; YEL019C; YEL019C; Saccharomyces cerevisiae. [Genome view]
GeneID	856695.
GenomeReviews	Gene locus YEL019C in contig U00092_GR.
KEGG	sce:YEL019C.
NMPDR	fig|4932.3.peg.1951.
Organism-specific databases
CYGD	YEL019c.
SGD	S000000745. MMS21.
Phylogenomic databases
HOGENOM	P38632.
OMA	CPITCKP.
Gene expression databases
ArrayExpress	P38632.
Genevestigator	P38632.
GermOnline	YEL019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004181. Znf_MIZ. [Graphical view]
PROSITE	PS51044. ZF_SP_RING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982748.

Entry information

Entry name

NSE2_YEAST

Accession

Primary (citable) accession number: P38632

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	September 27, 2004
Last modified:	November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)