Reviewed, UniProtKB/Swiss-Prot P36047 (SDS22_YEAST)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1 regulatory subunit SDS22

Gene names

Name:	SDS22
Synonyms:	EGP1
Ordered Locus Names:	YKL193C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potential regulator of the mitotic function of yeast type 1 protein phosphatase.
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SDS22 family. Contains 8 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from genetic interaction. Source: SGD maintenance of protein location in nucleus Inferred from genetic interaction. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 1 regulator activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-16783,EBI-2042717
GLC7	P32598	1	EBI-16783,EBI-13715
PPQ1	P32945	1	EBI-16783,EBI-13787
RNR2	P09938	1	EBI-16783,EBI-15240
SIC1	P38634	1	EBI-16783,EBI-17127
YPI1	P43587	1	EBI-16783,EBI-22913

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Protein phosphatase 1 regulatory subunit SDS22		PRO_0000071447
Regions
	Repeat	65 – 89	25	LRR 1
	Repeat	91 – 112	22	LRR 2
	Repeat	113 – 136	24	LRR 3
	Repeat	156 – 179	24	LRR 4
	Repeat	181 – 202	22	LRR 5
	Repeat	203 – 226	24	LRR 6
	Repeat	246 – 270	25	LRR 7
	Repeat	295 – 318	24	LRR 8
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P36047-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 1EF434C7276ABAEA	FASTA	338	38,887
	10 20 30 40 50 60 MDKNSVNKDS EEKDERHKIE VVDDTNPDFI TADSELTQDL PDDVEVIDLV HLKIKSLEDL 70 80 90 100 110 120 NLYRFKNLKQ LCLRQNLIES ISEVEVLPHD KIVDLDFYDN KIKHISSNVN KLTKLTSLDL 130 140 150 160 170 180 SFNKIKHIKN LENLTDLENL YFVQNSISKI ENLSTLKSLK NLELGGNKVH SIEPDSFEGL 190 200 210 220 230 240 SNLEEIWLGK NSIPRLINLH PLKNLKILSI QSNKLKKIEN LEELTNLEEL YLSHNFITKI 250 260 270 280 290 300 EGLEKNLKLT TLDVTSNKIT SLENLNHLSN LTDIWASFNK IDQSFESLGE NLSALSRLET 310 320 330 IYLEGNPIQL ENKTSYRRKL TMNLPPSLQK IDATYIRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase." Mackelvie S.H., Andrews P.D., Stark M.J.R. Mol. Cell. Biol. 15:3777-3785(1995) [PubMed: 7791785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200358 / YNN 295.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae." Cheret G., Mattheakis L.C., Sor F. Yeast 9:661-667(1993) [PubMed: 8394042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-136. Strain: S288c / GRF88.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83609 Genomic DNA. Translation: CAA58588.1. Z28193 Genomic DNA. Translation: CAA82037.1. AY558314 Genomic DNA. Translation: AAS56640.1. X69765 Genomic DNA. No translation available.
PIR	S38030.
RefSeq	NP_012728.1.
3D structure databases
HSSP	HSSP built from PDB template 1DS9 based on UniProtKB Q9XHH2.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2378N.
IntAct	P36047. 30 interactions.
STRING	P36047.
Proteomic databases
PeptideAtlas	P36047.
PRIDE	P36047.
Genome annotation databases
Ensembl	YKL193C; YKL193C; YKL193C; Saccharomyces cerevisiae. [Genome view]
GeneID	853641.
GenomeReviews	Gene locus YKL193C in contig Y13137_GR.
KEGG	sce:YKL193C.
NMPDR	fig|4932.3.peg.3705.
Organism-specific databases
CYGD	YKL193c.
SGD	S000001676. SDS22.
Phylogenomic databases
HOGENOM	P36047.
OMA	EFWMNDN.
Gene expression databases
ArrayExpress	P36047.
Genevestigator	P36047.
GermOnline	YKL193C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. [Graphical view]
Pfam	PF00560. LRR_1. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974534.

Entry information

Entry name

SDS22_YEAST

Accession

Primary (citable) accession number: P36047

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)