Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P36029 (TPO5_YEAST)

Last modified November 3, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Polyamine transporter TPO5
Gene names
Name: TPO5
Ordered Locus Names: YKL174C
ORF Names: YKL639
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length618 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Required for polyamine transport. Transports putrescine effectively and spermidine less effectively. Ref.4

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein. Ref.4

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 618618Polyamine transporter TPO5
PRO_0000054165

Regions

Topological domain1 – 6060Cytoplasmic Potential
Transmembrane61 – 8424 Potential
Topological domain85 – 906Extracellular Potential
Transmembrane91 – 11020 Potential
Topological domain111 – 13121Cytoplasmic Potential
Transmembrane132 – 14817 Potential
Topological domain149 – 1546Extracellular Potential
Transmembrane155 – 17117 Potential
Topological domain172 – 1798Cytoplasmic Potential
Transmembrane180 – 20021 Potential
Topological domain201 – 21111Extracellular Potential
Transmembrane212 – 23120 Potential
Topological domain232 – 29766Cytoplasmic Potential
Transmembrane298 – 31720 Potential
Topological domain318 – 34225Extracellular Potential
Transmembrane343 – 36725 Potential
Topological domain368 – 40235Cytoplasmic Potential
Transmembrane403 – 41917 Potential
Topological domain420 – 4256Extracellular Potential
Transmembrane426 – 44924 Potential
Topological domain450 – 46415Cytoplasmic Potential
Transmembrane465 – 48622 Potential
Topological domain487 – 49812Extracellular Potential
Transmembrane499 – 51618 Potential
Topological domain517 – 618102Cytoplasmic Potential

Amino acid modifications

Modified residue5371Phosphoserine Ref.6
Modified residue5691Phosphoserine Ref.6 Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P36029-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 035CB927C624CE74

FASTA61869,243
        10         20         30         40         50         60 
MPEYTLLADN IRENIVHFDP NGLFDNLHTI VHEDDSQENE EAEHFNYDQV LDKSLLSRGS 

        70         80         90        100        110        120 
IVGLGLGLMS PVLGMCTSMA IGLINGGPLT IMLGFLISGV CIWFSSLSLG EIVSKFPMEL 

       130        140        150        160        170        180 
HVGSAMLAPE KLKLVCSWYT GWLMLIGNWT MSTSITFAGA QLTISLILMT NSNLISEAHL 

       190        200        210        220        230        240 
IFYTVIVFYL VVTVVGLVNL KFARFIETIN KVCVYWIIYA IIFIDILLLV FHKGKFRSLK 

       250        260        270        280        290        300 
YALFHFDNNL SGYKSAFLSF IIGFQQSNFT LQGFSMLPAL ADEVKVPEKD IPRGMSNAVL 

       310        320        330        340        350        360 
LSAFSGVIFL IPIMLILPDN DLLFTNHKVL PIVNIFTKST DSVVLSFFLV LLILGNLLFS 

       370        380        390        400        410        420 
GIGSITTSSR AVYSFSRDQA IPYYDKWTYV EPDSQSKVPK NSVVLSMIIS YFLGLLALIS 

       430        440        450        460        470        480 
TAAFNAFIGA AVLCLCSATF IPLVLVLFTR RRAIRSAPVK IRYKFGWFIN IVSIVWLLLS 

       490        500        510        520        530        540 
MVSVCLPTQV PVTFKTMNYA LMVYVFCILV ITGLYFKWGK YNFRLPLADD IKAPIPSDAE 

       550        560        570        580        590        600 
ETVFELEDSN VEHTLNSGTT VKESVENNSE EGFIKVHPKS STENPFEENE ENVITDYGDE 

       610 
HHTAEQEFDL ADDRRYDI

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-569, MASS SPECTROMETRY.
[4]"Excretion of putrescine and spermidine by the protein encoded by YKL174c (TPO5) in Saccharomyces cerevisiae."
Tachihara K., Uemura T., Kashiwagi K., Igarashi K.
J. Biol. Chem. 280:12637-12642(2005) [PubMed: 15668236] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537 AND SER-569, MASS SPECTROMETRY.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

Z26878 Genomic DNA. Translation: CAA81512.1.
Z28174 Genomic DNA. Translation: CAA82016.1.
PIRS38004.
RefSeqNP_012747.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4038N.
IntActP36029. 4 interactions.
STRINGP36029.

Protein family/group databases

TCDB2.A.3.4.5. amino acid-polyamine-organocation (APC) family.

Genome annotation databases

EnsemblYKL174C; YKL174C; YKL174C; Saccharomyces cerevisiae. [Genome view]
GeneID853680.
GenomeReviewsGene locus YKL174C in contig Y13137_GR.
KEGGsce:YKL174C.
NMPDRfig|4932.3.peg.3725.

Organism-specific databases

CYGDYKL174c.
SGDS000001657. TPO5.

Phylogenomic databases

HOGENOMP36029.
OMATGVATIC.

Gene expression databases

ArrayExpressP36029.
GenevestigatorP36029.
GermOnlineYKL174C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
IPR004841. Permease_region.
[Graphical view]
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974640.

Hide | Top

Entry information

Entry nameTPO5_YEAST
AccessionPrimary (citable) accession number: P36029
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 3, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents