Reviewed, UniProtKB/Swiss-Prot P33563 (MTBB_BACSU)

Names and origin

Protein names

Recommended name:
    Modification methylase BsuBI
      Short name=M.BsuBI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase BsuBI

Gene names

Name:	hsdBM
Synonyms:	hsmB

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Modification methylase BsuBI		PRO_0000087946

Sequences

	Sequence		Length	Mass (Da)
	P33563-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: AC33B2944699653E	FASTA	501	57,198
	10 20 30 40 50 60 MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA KVLDAGAGIG 70 80 90 100 110 120 SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF KDYIEINSQI IIDDFIEWAA 130 140 150 160 170 180 YSLLDEESLL AKDKQRFTHA ILNPPYKKIK SNSKHRKLLR KAGIETVNLY SAFVALTVDL 190 200 210 220 230 240 MSDGGEIVFI IPRSFCNGPY FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK 250 260 270 280 290 300 LEKGTVQEDV KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV 310 320 330 340 350 360 CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP KMMKKPNAII 370 380 390 400 410 420 RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV NDPVVGFENG LNVLHYNKSG 430 440 450 460 470 480 ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ VNATDLRTMK FPSRDILISL GKWVIENIEN 490 500 VGQVEIDSKL EELLLNDRGN A

References

[1]	"BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes." Xu G.-L., Kapfer W., Walter J., Trautner T.A. Nucleic Acids Res. 20:6517-6523(1992) [PubMed: 1480472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ISB8.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01541 Genomic DNA. Translation: AAA18169.1.
PIR	S35515.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3336. M.BsuBI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 150.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBB_BACSU

Accession

Primary (citable) accession number: P33563

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	September 22, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)