Reviewed,
UniProtKB/Swiss-Prot P32368 (SAC1_YEAST)
Last modified
November 3, 2009.
Version 78.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Phosphoinositide phosphatase SAC1 EC=3.1.3.- Alternative name(s): Recessive suppressor of secretory defect | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 623 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P. Has low activity towards PtdIns(3,5)P2. May be involved in the coordination of the activities of the secretory pathway and the actin cytoskeleton. Ref.5 Ref.6 |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein. Ref.6 |
| Miscellaneous | Present with 48000 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Contains 1 SAC domain. |
| Caution | It is uncertain whether Met-1 or Met-59 is the initiator. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | phosphoinositide dephosphorylation Ref.5 Inferred from direct assay. Source: SGD |
| Cellular component | integral to Golgi membrane Inferred from direct assay. Source: SGD integral to endoplasmic reticulum membrane Ref.6Inferred from direct assay. Source: SGD mitochondrial outer membraneInferred from direct assay. Source: SGD |
| Molecular function | identical protein binding Inferred from physical interaction. Source: IntAct phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity Ref.5Inferred from direct assay. Source: SGD phosphatidylinositol-3-phosphatase activity Ref.5Inferred from direct assay. Source: SGD phosphatidylinositol-4-phosphate phosphatase activity Ref.5Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 623 | 623 | Phosphoinositide phosphatase SAC1 | PRO_0000209737 | |||||
Regions | |||||||||
| Topological domain | 1 – 521 | 521 | Extracellular Potential | ||||||
| Transmembrane | 522 – 544 | 23 | Potential | ||||||
| Topological domain | 545 – 623 | 79 | Cytoplasmic Potential | ||||||
| Domain | 115 – 454 | 340 | SAC | ||||||
| Compositional bias | 507 – 521 | 15 | Pro-rich | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function." Cleves A.E., Novick P.J., Bankaitis V.A. J. Cell Biol. 109:2939-2950(1989) [PubMed: 2687291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.  Mewes H.-W.Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [4] | Hochstrasser M., Gang G. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-145. |
| [5] | "SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases." Hughes W.E., Woscholski R., Cooke F.T., Patrick R.S., Dove S.K., McDonald N.Q., Parker P.J. J. Biol. Chem. 275:801-808(2000) [PubMed: 10625610] [Abstract] Cited for: FUNCTION. |
| [6] | "Sac1 lipid phosphatase and Stt4 phosphatidylinositol 4-kinase regulate a pool of phosphatidylinositol 4-phosphate that functions in the control of the actin cytoskeleton and vacuole morphology." Foti M., Audhya A., Emr S.D. Mol. Biol. Cell 12:2396-2411(2001) [PubMed: 11514624] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X51672 Genomic DNA. Translation: CAA35979.1. X75951 Genomic DNA. Translation: CAA53561.1. Z28212 Genomic DNA. Translation: CAA82057.1. U39947 Genomic DNA. Translation: AAA82257.1. | |
| PIR | A33622. |
| RefSeq | NP_012710.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6610N. |
| IntAct | P32368. 131 interactions. |
| STRING | P32368. |
Proteomic databases | |
| PeptideAtlas | P32368. |
Genome annotation databases | |
| Ensembl | YKL212W; YKL212W; YKL212W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 853668. |
| GenomeReviews | Gene locus YKL212W in contig Y13137_GR. |
| KEGG | sce:YKL212W. |
| NMPDR | fig|4932.3.peg.3688. |
Organism-specific databases | |
| CYGD | YKL212w. |
| SGD | S000001695. SAC1. |
Phylogenomic databases | |
| HOGENOM | P32368. |
| OMA | YVNWPKL. |
Gene expression databases | |
| ArrayExpress | P32368. |
| Genevestigator | P32368. |
| GermOnline | YKL212W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002013. Syja_N. [Graphical view] |
| Pfam | PF02383. Syja_N. 1 hit. [Graphical view] |
| PROSITE | PS50275. SAC. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 974608. |
Entry information
| Entry name | SAC1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32368 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XI Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names |
