Reviewed, UniProtKB/Swiss-Prot P19888 (MTBA_BACAR)

Names and origin

Protein names

Recommended name:
    Modification methylase BanI
      Short name=M.BanI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase BanI

Gene names

Name:

banIM

Organism

Bacillus aneurinolyticus

Taxonomic identifier

1391 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Aneurinibacillus group › Aneurinibacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGYRCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BanI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Modification methylase BanI		PRO_0000087860
Sites
	Active site	76	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19888-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: ADF3E932A681E43E	FASTA	428	48,618
	10 20 30 40 50 60 MKIKFVDLFA GIGGIRIGFE RAAKRFELET ECVLSSEIDK KACETYALNF KEEPQGDIHE 70 80 90 100 110 120 ITSFPEFDFL LAGFPCQPFS YAGKQQGFGD TRGTLFFEVE RVLRDNRPKA FLLENVRGLV 130 140 150 160 170 180 THDKGRTLKT IISKLEELGY GVSYLLLNSS TFGVPQNRVR IYILGILGSK PKLTLTSNVG 190 200 210 220 230 240 AADSHKYKNE QISLFDESYA TVKDILEDSP SEKYRCSDEF IGQLSKVVGN NFELLHGYRL 250 260 270 280 290 300 IDYRGGNSIH SWELGIKGDC TKEEIEFLNQ LIANRRKKIY GTHQDGKALT LEQIRTFYNH 310 320 330 340 350 360 DQLEVIIKSL LQKGYLREEE NKFNPVCGNM SFEVFKFLDP DSISITLTSS DAHKLGVVQN 370 380 390 400 410 420 NVPRRITPRE CARLQGFPDD FILHSNDNFA YKQLGNSVTV KVVEKVIEDL FQNNVNELFG QMKLANVV

References

[1]	"Cloning and nucleotide sequences of the BanI restriction-modification genes in Bacillus aneurinolyticus." Maekawa Y., Yasukawa H., Kawakami B. J. Biochem. 107:645-649(1990) [PubMed: 2358438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: IAM 1077.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00704 Genomic DNA. Translation: BAA00613.1.
PIR	CTBSBA. JS0489.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.37. 142380.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBA_BACAR

Accession

Primary (citable) accession number: P19888

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	July 7, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)