Reviewed, UniProtKB/Swiss-Prot P13298 (PYRE_YEAST)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase 1
      Short name=OPRTase 1
      Short name=OPRT 1
    EC=2.4.2.10

Gene names

Name:	URA5
Synonyms:	PYR5
Ordered Locus Names:	YML106W
ORF Names:	YM8339.13

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Subunit structure	Homodimer By similarity.
Miscellaneous	There are two genes coding for OPRT in yeast. Present with 39300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from mutant phenotype. Source: SGD pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine ribonucleoside biosynthetic process Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	orotate phosphoribosyltransferase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P36076	1	EBI-14392,EBI-26778
PPZ1	P26570	1	EBI-14392,EBI-13807

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 226

226

Orotate phosphoribosyltransferase 1

PRO_0000110804

Regions

Region

38 – 39

2

Orotate binding By similarity

Region

76 – 77

2

5-phosphoribose 1-diphosphate binding By similarity

Region

132 – 140

9

5-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site

30

1

5-phosphoribose 1-diphosphate By similarity

Binding site

106

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

107

1

5-phosphoribose 1-diphosphate By similarity

Binding site

110

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

112

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

136

1

Orotate By similarity

Binding site

164

1

Orotate By similarity

Experimental info

Sequence conflict

150

1

N → S in CAA32901. Ref.1

Secondary structure

1

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226

Helix Strand Turn

Details...

Helix

7 – 18

12

Beta strand

21 – 28

8

Beta strand

34 – 39

6

Helix

41 – 43

3

Helix

47 – 63

17

Beta strand

69 – 73

5

Turn

75 – 77

3

Helix

78 – 92

15

Helix

95 – 97

3

Beta strand

101 – 105

5

Beta strand

118 – 121

4

Beta strand

127 – 131

5

Helix

141 – 150

10

Beta strand

154 – 163

10

Beta strand

166 – 169

4

Helix

178 – 186

9

Beta strand

190 – 195

6

Helix

196 – 202

7

Helix

211 – 222

12

Sequences

	Sequence		Length	Mass (Da)
	P13298-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: ECF3A01EB35BC03F	FASTA	226	24,664
	10 20 30 40 50 60 MPIMLEDYQK NFLELAIECQ ALRFGSFKLK SGRESPYFFN LGLFNTGKLL SNLATAYAIA 70 80 90 100 110 120 IIQSDLKFDV IFGPAYKGIP LAAIVCVKLA EIGGSKFQNI QYAFNRKEAK DHGEGGIIVG 130 140 150 160 170 180 SALENKRILI IDDVMTAGTA INEAFEIISN AKGQVVGSII ALDRQEVVST DDKEGLSATQ 190 200 210 220 TVSKKYGIPV LSIVSLIHII TYLEGRITAE EKSKIEQYLQ TYGASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the URA5 gene of Saccharomyces cerevisiae." de Montigny J., Belarbi A., Hubert J.-C., Lacroute F. Mol. Gen. Genet. 215:455-462(1989) [PubMed: 2651891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Stirling C.J. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-226.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X14795 Genomic DNA. Translation: CAA32901.1.
Z49210 Genomic DNA. Translation: CAA89112.1.
AY693160 Genomic DNA. Translation: AAT93179.1.
X65783 Genomic DNA. Translation: CAA46665.1.

PIR

XJBY5. S53966.

RefSeq

NP_013601.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PRY	X-ray	2.35	A	1-226	[»]
2PRZ	X-ray	1.90	A/B/C/D	1-226	[»]
2PS1	X-ray	1.75	A/B	1-226	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4859N.

IntAct

P13298. 6 interactions.

STRING

P13298.

Proteomic databases

PeptideAtlas

P13298.

Genome annotation databases

Ensembl

YML106W; YML106W; YML106W; Saccharomyces cerevisiae. [Genome view]

GeneID

854865.

GenomeReviews

Gene locus YML106W in contig Z71257_GR.

KEGG

sce:YML106W.

NMPDR

fig|4932.3.peg.4637.

Organism-specific databases

CYGD

YML106w.

SGD

S000004574. URA5.

Phylogenomic databases

HOGENOM

P13298.

OMA

YKGITLA.

Enzyme and pathway databases

BRENDA

2.4.2.10. 250.

Gene expression databases

ArrayExpress

P13298.

Genevestigator

P13298.

GermOnline

YML106W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004467. Or_phspho_trans.
IPR002375. Pr/py_Pribosyl_transf_CS.
IPR000836. PRibTrfase.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00336. pyrE. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

977789.

Entry information

Entry name

PYRE_YEAST

Accession

Primary (citable) accession number: P13298

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)