Reviewed, UniProtKB/Swiss-Prot P06529 (T2BR_BACSU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme BsuRI
      Short name=R.BsuRI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme BsuRI
    Endonuclease BsuRI

Gene names

Name:	hsdRR
Synonyms:	hsrR

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGCC and cleaves after G-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Monomer.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Type-2 restriction enzyme BsuRI		PRO_0000077292

Sequences

	Sequence		Length	Mass (Da)
	P06529-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 054D1182E510E12F	FASTA	576	66,304
	10 20 30 40 50 60 MGKNSKAIGN NHVKSVYQAL LQSLKSKSVN GFSKITIETI SFIKNLYPEI DSVTSKFDNS 70 80 90 100 110 120 RPDQSKDLTL YLKSGETISL NLFLIKKGRR IQPKNAGAKS FLEKYFLSAE MQKIFNKEFE 130 140 150 160 170 180 RYYLDYLKEV VEHKKGTHYI TDKRELKRLV SSHFPKFTEE INLYRDKFLF NLRETCFTLL 190 200 210 220 230 240 QQFYNEKNIG FTHAFNVFFM VNDTNIITSY GKDENDVKVE KFAPASPSLK DIELYKTGKS 250 260 270 280 290 300 TVGIKFGEVG LTLRFKFESD PWKSIKLATG YHEFPKEKER VNVNLKTMRR MEKLLNKHEY 310 320 330 340 350 360 AKTSNNSNAI GKCHEAWTYY YFLKAFPDVI QVDPKQCVEL INTYFSSINQ NTLKKLYSST 370 380 390 400 410 420 STIVDAITEK LRQKYHDYII ESIELIPDAY IKDRLDTGDL QLVLKVNNNI IVENISLKAL 430 440 450 460 470 480 AKRNSKITTK NPGMGSILGP TYFNMGSMES VINEVKNKFT IGEFNHRKSL EILSYEFGMK 490 500 510 520 530 540 LDSATQEQLR RGIHNLLGKA MIAITIYGEG ISFCKEPSEI DGEVKVHVNV PSAIQNTLTW 550 560 570 NNELESISLR AKFSKSQKHG WSSIKLTSEC QLESRK

References

[1]	"Nucleotide sequence of the BsuRI restriction-modification system." Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J. Nucleic Acids Res. 13:6403-6421(1985) [PubMed: 2997708] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02988 Genomic DNA. Translation: CAA26730.1.
PIR	NDBSR1. A23488.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	620. BsuRI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 150.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2BR_BACSU

Accession

Primary (citable) accession number: P06529

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 20, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)