Reviewed, UniProtKB/Swiss-Prot P05986 (KAPC_YEAST)

Names and origin

Protein names

Recommended name:
    cAMP-dependent protein kinase type 3
      Short name=PKA 3
    EC=2.7.11.11

Gene names

Name:	TPK3
Synonyms:	PKA3
Ordered Locus Names:	YKL166C
ORF Names:	YKL630

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by cAMP.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding cAMP
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Ras protein signal transduction Ref.1 Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cAMP-dependent protein kinase complex Ref.1 Inferred from genetic interaction. Source: SGD cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCY1	P07278	1	EBI-9470,EBI-9475

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	cAMP-dependent protein kinase type 3		PRO_0000086071
Regions
	Domain	88 – 342	255	Protein kinase
	Domain	343 – 398	56	AGC-kinase C-terminal
	Nucleotide binding	94 – 102	9	ATP By similarity
Sites
	Active site	211	1	Proton acceptor By similarity
	Binding site	117	1	ATP By similarity
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.7 Ref.8
	Modified residue	23	1	Phosphothreonine Ref.9
	Modified residue	37	1	Phosphoserine Ref.8
	Modified residue	242	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	4	1	D → E in AAA35166. Ref.1
	Sequence conflict	208	1	I → T in AAA35166. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05986-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 4ADDE8E63032B313	FASTA	398	45,977
	10 20 30 40 50 60 MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI NGRNSGKLKE 70 80 90 100 110 120 EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG RVHLIRSNHN GRFYALKTLK 130 140 150 160 170 180 KHTIVKLKQV EHTNDERRML SIVSHPFIIR MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS 190 200 210 220 230 240 QRFPNPVAKF YAAEVCLALE YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT 250 260 270 280 290 300 YTLCGTPDYI APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL 310 320 330 340 350 360 KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL LARYIETPYE 370 380 390 PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase." Toda T., Cameron S., Sass P., Zoller M., Wigler M. Cell 50:277-287(1987) [PubMed: 3036373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Structure and function of MRP20 and MRP49, the nuclear genes for two proteins of the 54 S subunit of the yeast mitochondrial ribosome." Fearon K., Mason T.L. J. Biol. Chem. 267:5162-5170(1992) [PubMed: 1544898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 344-398.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-242, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-37, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17074 Genomic DNA. Translation: AAA35166.1. Z26878 Genomic DNA. Translation: CAA81521.1. Z28166 Genomic DNA. Translation: CAA82008.1. M81697 Genomic DNA. Translation: AAA34791.1.
PIR	OKBYC3. S37996.
RefSeq	NP_012755.1.
3D structure databases
HSSP	HSSP built from PDB template 1FOT based on UniProtKB P06244.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:550N.
IntAct	P05986. 16 interactions.
STRING	P05986.
Proteomic databases
PeptideAtlas	P05986.
PRIDE	P05986.
Genome annotation databases
Ensembl	YKL166C; YKL166C; YKL166C; Saccharomyces cerevisiae. [Genome view]
GeneID	853688.
GenomeReviews	Gene locus YKL166C in contig Y13137_GR.
KEGG	sce:YKL166C.
NMPDR	fig|4932.3.peg.3733.
Organism-specific databases
CYGD	YKL166c.
SGD	S000001649. TPK3.
Phylogenomic databases
HOGENOM	P05986.
OMA	HRETGQH.
Enzyme and pathway databases
BRENDA	2.7.11.11. 250.
Gene expression databases
ArrayExpress	P05986.
Genevestigator	P05986.
GermOnline	YKL166C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974661.

Entry information

Entry name

KAPC_YEAST

Accession

Primary (citable) accession number: P05986

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)