Reviewed, UniProtKB/Swiss-Prot P05648 (DNAA_BACSU)

Names and origin

Protein names

Recommended name:
    Chromosomal replication initiator protein dnaA

Gene names

Name:	dnaA
Synonyms:	dnaH
Ordered Locus Names:	BSU00010

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes. HAMAP MF_00377
Subunit structure	Some 20 dnaA protein molecules bind their sites in oriC. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication initiation Inferred from electronic annotation. Source: HAMAP regulation of DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA replication origin binding Inferred from electronic annotation. Source: HAMAP nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
soj	P37522	1	EBI-2014722,EBI-2014674

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Chromosomal replication initiator protein dnaA HAMAP MF_00377		PRO_0000114133
Regions
	Nucleotide binding	151 – 158	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P05648-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 666799A0A183C992	FASTA	446	50,859
	10 20 30 40 50 60 MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH 70 80 90 100 110 120 LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF 130 140 150 160 170 180 VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL 190 200 210 220 230 240 SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES 250 260 270 280 290 300 KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV 310 320 330 340 350 360 MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ 370 380 390 400 410 420 RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA 430 440 HEKISKLLAD DEQLQQHVKE IKEQLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region." Moriya S., Ogasawara N., Yoshikawa H. Nucleic Acids Res. 13:2251-2265(1985) [PubMed: 2987847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H. EMBO J. 7:2911-2917(1988) [PubMed: 2846289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446. Strain: 168 / PSL1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02369 Genomic DNA. Translation: CAA26217.1. D26185 Genomic DNA. Translation: BAA05237.1. AL009126 Genomic DNA. Translation: CAB11777.1. X12778 Genomic DNA. Translation: CAA31269.1. X12779 Genomic DNA. Translation: CAA31270.1.
PIR	IQBSOC. A22930.
RefSeq	NP_387882.1.
3D structure databases
HSSP	HSSP built from PDB template 1J1V based on UniProtKB P03004.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05648. 1 interaction.
Genome annotation databases
GeneID	939978.
GenomeReviews	Gene locus BSU00010 in contig AL009126_GR.
KEGG	bsu:BSU00010.
NMPDR	fig|224308.1.peg.1.
Organism-specific databases
SubtiList	BG10065. dnaA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P05648.
OMA	PPKEIAK.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0001-MON.
Family and domain databases
HAMAP	MF_00377. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. [Graphical view]
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BACSU

Accession

Primary (citable) accession number: P05648

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)