Reviewed, UniProtKB/Swiss-Prot P00472 (MTE1_ECOLX)

Names and origin

Protein names

Recommended name:
    Modification methylase EcoRI
      Short name=M.EcoRI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase EcoRI

Gene names

Name:

ecoRIM

Encoded on

Plasmid pMB1 Ref.1
Plasmid pMB4 Ref.2

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 326	325	Modification methylase EcoRI		PRO_0000087959

Sequences

	Sequence		Length	Mass (Da)
	P00472-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5F6AFE1DD1CBB1A8	FASTA	326	38,044
	10 20 30 40 50 60 MARNATNKLL HKAKKSKSDE FYTQYCDIEN ELQYYREHFS DKVVYCNCDD PRVSNFFKYF 70 80 90 100 110 120 AVNFDNLGLK KLIASCYVEN KEGFSSSEAA KNGFYYEYHK ENGKKLVFDD ISVSSFCGDG 130 140 150 160 170 180 DFRSSESIDL LKKSDIVVTN PPFSLFREYL DQLIKYDKKF LIIANVNSIT YKEVFNLIKE 190 200 210 220 230 240 NKIWLGVHLG RGVSGFIVPE HYELYGTEAR IDSNGNRIIS PNNCLWLTNL DVFIRHKDLP 250 260 270 280 290 300 LTRKYFGNES SYPKYDNYDA INVNKTKDIP LDYNGVMGVP ITFLHKFNPE QFELIKFRKG 310 320 VDEKDLSING KCPYFRILIK NKRLQK

References

[1]	"Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase." Greene P.J., Gupta M., Boyer H.W., Brown W.E., Rosenberg J.M. J. Biol. Chem. 256:2143-2153(1981) [PubMed: 6257703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes." Newman A.K., Rubin R.A., Kim S.-H., Modrich P. J. Biol. Chem. 256:2131-2139(1981) [PubMed: 6257701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[3]	"Partial NH2- and COOH-terminal sequence analyses of Eco RI DNA restriction and modification enzymes." Rubin R.A., Modrich P., Vanaman T.C. J. Biol. Chem. 256:2140-2142(1981) [PubMed: 6257702] [Abstract] Cited for: CONFIRMATION OF AMINO AND CARBOXYL ENDS OF SEQUENCE BY AMINO ACID ANALYSIS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01675 Genomic DNA. Translation: AAA26372.1.
PIR	XYECP4. A92308.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3395. M.EcoRI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 246.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTE1_ECOLX

Accession

Primary (citable) accession number: P00472

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)