Reviewed, UniProtKB/Swiss-Prot B3W7I3 (LACG_LACCB)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	LCABL_07280

Organism

Lactobacillus casei (strain BL23) [Complete proteome] [HAMAP]

Taxonomic identifier

543734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_1000147418
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3W7I3-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: EBE1B0C3A45498C9	FASTA	474	53,968
	10 20 30 40 50 60 MSKQLPQDFV MGGATAAYQV EGATKEDGKG RVLWDDFLDK QGRFKPDPAA DFYHRYDEDL 70 80 90 100 110 120 ALAEKYGHQV IRVSIAWSRI FPDGAGEVEP RGVAFYHKLF ADCAAHHIEP FVTLHHFDTP 130 140 150 160 170 180 ERLHEAGDWL SQEMLDDFVA YAKFCFEEFS EVKYWITINE PTSMAVQQYT SGTFPPAESG 190 200 210 220 230 240 RFDKTFQAEH NQMVAHARIV NLYKSMQLGG QIGIVHALQT VYPYSDSAVD HHAAELQDAL 250 260 270 280 290 300 ENRLYLDGTL AGEYHQETLA LVKEILDANH QPMFQSTPQE MKAIDEAAHQ LDFVGVNNYF 310 320 330 340 350 360 SKWLRAYHGK SETIHNGDGT KGSSVARLQG VGEEKLPDGI ETTDWDWSIY PRGMYDILMR 370 380 390 400 410 420 IHNDYPLVPV TYVTENGIGL KESLPENATP DTVIEDPKRI DYVKKYLSAM ADAIHDGANV 430 440 450 460 470 KGYFIWSLQD QFSWTNGYSK RYGLFFVDFP TQNRYIKQSA EWFKSVSETH IIPD

References

[1]	"Lactobacillus casei BL23 complete genome sequence." Maze A., Boel G., Bourand A., Loux V., Gibrat J.F., Zuniga M., Hartke A., Deutscher J. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM177140 Genomic DNA. Translation: CAQ65853.1.
RefSeq	YP_001986711.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6406104.
GenomeReviews	Gene locus LCABL_07280 in contig FM177140_GR.
KEGG	lcb:LCABL_07280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TKYPLDP.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_LACCB

Accession

Primary (citable) accession number: B3W7I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)