Reviewed, UniProtKB/Swiss-Prot A9NGN9 (SYA_ACHLI)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ACL_0906

Organism

Acholeplasma laidlawii (strain PG-8A) [Complete proteome] [HAMAP]

Taxonomic identifier

441768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Acholeplasma

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347467

Sequences

	Sequence		Length	Mass (Da)
	A9NGN9-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 474CFC83A56C418A	FASTA	851	96,289
	10 20 30 40 50 60 MTSEQIRQTW LNFFKDKGHI IEPSASLIPV DDPTLLWINA GVAPLKKYFD GTETPKSKRI 70 80 90 100 110 120 TNIQKSIRTN DIDNVGKTAR HHTFFEMMGN FSIGDYFKKE AIEFGFELLT SPKYFDIPLD 130 140 150 160 170 180 KLYMTYYPTD VEAKNTWLKL GVKEDHLIPV EGNFWEIGAG PSGPDTEIFF DRGTSYDLRG 190 200 210 220 230 240 PELIRDDIEN ERFIEIWNIV FSQYNADPKL KRSEYKELPN KNIDTGAGLE RFACVLQGTK 250 260 270 280 290 300 TNFETDLHYP VILKVESLSG VKYDGQMAFK VISDHIKTLV FAISDGANLS NEGRGYVLRR 310 320 330 340 350 360 ILRRAVKYGK SIGFDAPFLH LLVDTVVDMM SNFYTNLIET AVIVKKIILK EEEKFFSTIL 370 380 390 400 410 420 DGEKHLLQSI KDGKLSGEDA FKLYDTYGFP IELTIEYAEE HGITVDIKGF NEALEVQKSR 430 440 450 460 470 480 SRDARKVTHS MKGQDEALLN FDVPSEFVGY DYLQTESKVI KVFDQGIVLD KTPFYANSGG 490 500 510 520 530 540 QISDTGSING LVVRDVIKLP HGQHLHLIET DAFTEGDEVL CIVNQDKRHH TMKNHTATHL 550 560 570 580 590 600 LNQALKDTLG SHVKQQGSYN GDLKLTFDIN HYETIKDEEI LTVEKIVQQK IKEQIDVVTH 610 620 630 640 650 660 VLPIDEAKKL GAAMQFGEKY GDIVRVVDVG SWSMEFCGGT HVKNTSDIKN FMITSVESIG 670 680 690 700 710 720 SGTYRFEAIT GQVLSQVKKI VQNLLDMIHQ HIEKIKSLDE NHNLKPLPEL TGSYQDIINL 730 740 750 760 770 780 RLYNQYISNE SKVVEKQLEA KLIDQIILQA DTFIPKTIEP TTYIYSDNLP QSSLKPLIDV 790 800 810 820 830 840 LYDKIKADTV VLLNISEDKA SYLVKSSVNE ARNVINELNK VLDGRGGGKP DFAQGGTQAL 850 DKVQMFLKGK N

References

[1]

"Acholeplasma laidlawii complete genome." Kovaleva G.Y., Kazanov M.D., Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A., Vereshchagin V.A., Kostrjukova E.S., Selezneva O.V., Vtyurin N.N., Rogov S.I., Alekseev D.G., Ladygina V.G. , Titova G.A., Karpov V.A., Govorun V.M. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000896 Genomic DNA. Translation: ABX81519.1.
RefSeq	YP_001620895.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5803737.
GenomeReviews	Gene locus ACL_0906 in contig CP000896_GR.
KEGG	acl:ACL_0906.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ASGKHND.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACHLI

Accession

Primary (citable) accession number: A9NGN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)