Reviewed, UniProtKB/Swiss-Prot A8ICW8 (SYA_AZOC5)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AZC_2854

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347496
Regions
	Zinc finger	169 – 182	14	C2H2-type HAMAP MF_00036

Sequences

	Sequence		Length	Mass (Da)
	A8ICW8-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C967993014278427	FASTA	881	94,871
	10 20 30 40 50 60 MSGVNEIRSS FIDYFVKNGH EAVASSPLVP RNDPTLMFTN AGMVQFKNVF TGVEKRPYSR 70 80 90 100 110 120 AVTAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKDRAIELA WNLITKEWDL 130 140 150 160 170 180 PKDRLLATVY YDDDVAYDLW KKVAGLPDSR IIRIPTSDNF WAMGDTGPCG PCSEIFFDHG 190 200 210 220 230 240 DHIPGGPPGS PDEDGDRFIE IWNLVFMQYE QLPGERLNLP RPSIDTGMGL ERISALLQGT 250 260 270 280 290 300 HDNYETDLMR AIIAEVQELT NVPSNGPQKA SHRVIADHLR SSVFLVADGV LPSNEGRGYV 310 320 330 340 350 360 LRRIMRRAMR HAQLLGAREP LMHRLVPVLV REMGRAYPEI VRAEALATET LLLEETRFRR 370 380 390 400 410 420 TLERGLSLLE EESAGLVSGA RFPGEVAFKL YDTYGFPLDL TEDALRGRGI EVEREAFDAA 430 440 450 460 470 480 MERQKAEARA NWSGSGEAAT DTVWFGVRER EGATEFLGYD TETAEGAVRA LVQEGKEVSE 490 500 510 520 530 540 LPAGARGFVV LNQTPFYGES GGQVGDSGLV SGEGVKACVL NTQKKLGDVF VHDVEVTEGT 550 560 570 580 590 600 LKIGTPLALE VDHARRSAVR ANHSATHLLH EALRRVLGDH VAQKGSLVAP DRLRFDFSHP 610 620 630 640 650 660 KPLTADELAQ VEDIANTYVL RNEPVETRLM AVDDAVASGA RALFGEKYGD EVRVVSMGTD 670 680 690 700 710 720 SGNGAPFSVE LCGGTHVKRT GDIGIVTVLA DSGVAAGVRR LEALTATAAR RHLNAASTAL 730 740 750 760 770 780 QTTAGTLKVS TAEVEARVAA LVEERRKLER DLAEARKKLA MGGGGESGPA VIEVNGTRYL 790 800 810 820 830 840 RIAISGADPK DLKAIVDEGK TKIGSGVVAV ANTAADGKGA LVVGVTADLA GKFDAQKLVR 850 860 870 880 IGVEVLGGKG GGGRPDMAQG GGPDGAKADA ALAAIESALG A

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF88852.1.
RefSeq	YP_001525770.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5688279.
GenomeReviews	Gene locus AZC_2854 in contig AP009384_GR.
KEGG	azc:AZC_2854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AZOC5

Accession

Primary (citable) accession number: A8ICW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)