Reviewed, UniProtKB/Swiss-Prot A8FFM9 (SYA_BACP2)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BPUM_2380

Organism

Bacillus pumilus (strain SAFR-032) [Complete proteome] [HAMAP]

Taxonomic identifier

315750 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347499

Sequences

	Sequence		Length	Mass (Da)
	A8FFM9-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 4B26A30C1E8700CC	FASTA	878	97,040
	10 20 30 40 50 60 MKTLTSAQVR QMFLDFFKEK GHAVEPSASL VPHDDPTLLW INSGVATLKK YFDGRVVPDN 70 80 90 100 110 120 PRICNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAIEWAWE FLTSDEWIGF 130 140 150 160 170 180 DPNLLSVTVH PEDEEAYVLW RDKIGVPEER IIRLEGNFWD IGEGPSGPNT EIFYDRGESY 190 200 210 220 230 240 GHDMNDPELY PGGENERYLE VWNLVFSEFN HNPDGSYTPL PKKNIDTGMG LERMVSVIQN 250 260 270 280 290 300 VPTNFDTDLF MPIIRAVETI SGESYGETKE KDTAFKVIAD HIRTVAFAVS DGALPSNEGR 310 320 330 340 350 360 GYVLRRLLRR AVRYAKTIHI HRPFMFDLVP VVAEIMKDFY PDVQAKEEFI AKVIKNEEER 370 380 390 400 410 420 FHETLNEGLA ILSEVIKKER DKGSSQISGE DVFKLYDTYG FPVELTEEYA EDEHMTVDRE 430 440 450 460 470 480 GFQAEMEKQR ERARNARQDV GSMQVQGGAL GDIKVESTFV GYENLTAVAH MIELLQNGEI 490 500 510 520 530 540 VSEAHEGDTV QILLDETPFY AESGGQVADK GTLKSAEVII DIKDVKKAPN GQHVHEGVVV 550 560 570 580 590 600 SGTAKKGLVV TAEVESALRK GIVKNHTATH LLHQALKDVL GSHVNQAGSL VNENRLRFDF 610 620 630 640 650 660 SHFGQVTKEE LSQIEKIVNE KIWEGISVAI DLKPIAEAKE MGAMALFGEK YGDIVRVVQV 670 680 690 700 710 720 GDYSIELCGG CHVQNTAEIG LFKIASESGI GAGTRRIEAV TGKGAYEELN DQLGILEQAA 730 740 750 760 770 780 SELKSNTKDV PKRIASLQAD LKEVQRENES LLAKLSQAEA GSILEKVTEI GGVKVLTEKV 790 800 810 820 830 840 NAKDMNHLRT MVDDLKAKLG SAVIVLGAVQ NGKVNISAGV TKDVIEKGLH AGKLVKQAAE 850 860 870 ICGGGGGGRP DMAQAGGKQP EKLEEALTSV EESVKSVL

References

[1]

"Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032." Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D., Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C. , Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K., Weinstock G.M. PLoS ONE 2:E928-E928(2007) [PubMed: 17895969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000813 Genomic DNA. Translation: ABV63046.1.
RefSeq	YP_001487606.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8FFM9.
Genome annotation databases
GeneID	5621649.
GenomeReviews	Gene locus BPUM_2380 in contig CP000813_GR.
KEGG	bpu:BPUM_2380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACP2

Accession

Primary (citable) accession number: A8FFM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)