Reviewed, UniProtKB/Swiss-Prot A1A198 (SYA_BIFAA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BAD_0700

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347507

Sequences

	Sequence		Length	Mass (Da)
	A1A198-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 302D8579893EFF45	FASTA	892	97,576
	10 20 30 40 50 60 MRTSEIAKRF LDYFEQHDHL VVPSASLISP NPTTLFTIAG MVPFIPYLMG EQTPPKHRMA 70 80 90 100 110 120 SNQKCVRTLD IDEVGKTTRH GTFFQMLGNF SFGDYFKEEA IHYAYELLTT PQDKGGYGFD 130 140 150 160 170 180 PEKLWMTTFT DDEEARSMWK NEGVDPEHIQ IMGMEDNFWT TGGPGPGGPC SEIYVDRGPK 190 200 210 220 230 240 YGVEGGPIAD ENRYIEIWDL VFENYEVDNV KSKTDLHIVG ELENKNIDTG AGLERLAYLM 250 260 270 280 290 300 QGKQNIYETD EVFPVIEAAQ KLSGRTYGDD EAMDVRFRIV ADHVRSALMI MSDGVRPSNN 310 320 330 340 350 360 GRGYVLRRLL RRTVKAMREL GVTEPVMPTL LPTSKAAMEP SYPELNDTFH DVSEAAYGEE 370 380 390 400 410 420 DAFRRTLESG TEIFDMAVTK AKQGGSDLVS GEDAFKLHDT YGFPIEITLE MAADQGVKVD 430 440 450 460 470 480 EAKFRELMAE QKSRARADAL KKRHNVDLSV YDDFKKTLLQ PIDFLGYTDM SARAKVLGIM 490 500 510 520 530 540 QEGKGSVPAV TGPANIEVIL DRTPFYAQAG GQLADQGEIL SDDGAVLEVD DVQKPIKDLI 550 560 570 580 590 600 VHQCRLTEGT LVVGAEVNAN IDLERRGAIA RSHTATHMVH KALREELGPQ ATQRGSEDAP 610 620 630 640 650 660 NRLRFDFQWS SAPSKDAMNA VEARVNEKLR ENLAVTTQEM KFDDAIALGA MHLFGEKYGD 670 680 690 700 710 720 IVRVVSIGED GWSRELCGGT HIDHVGKIGA INIMSEASIG SGVRRVDAVV GQGAYEFNAR 730 740 750 760 770 780 EHALVSQLSD MVNARPDELA ERVNMLLAKL KESDRRLAAM YESQLAASVP TLVAEAKASS 790 800 810 820 830 840 APVKVAKKNV GHFGSFDALR KTVLDVRGQL GEDAPVVVAL AGMNEDGKPM VAVATNEAAR 850 860 870 880 890 KQGIKAGDLV RGASKVLGGG GGGKPDFAQG GGADATKIDE ALEALAGQAL KG

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009256 Genomic DNA. Translation: BAF39481.1.
RefSeq	YP_909563.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1A198.
Genome annotation databases
GeneID	4557202.
GenomeReviews	Gene locus BAD_0700 in contig AP009256_GR.
KEGG	bad:BAD_0700.
NMPDR	fig|1680.3.peg.1278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BIFAA

Accession

Primary (citable) accession number: A1A198

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)