Reviewed, UniProtKB/Swiss-Prot A0KPG1 (SYA_AERHH)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AHA_3714

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347480
Regions
	Zinc finger	177 – 190	14	C2H2-type HAMAP MF_00036

Sequences

	Sequence		Length	Mass (Da)
	A0KPG1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 4D5F96148F865FA0	FASTA	874	95,942
	10 20 30 40 50 60 MYMSTSEIRT AFLEYFRSQG HQVVSSSSLV PHNDPTLLFT NAGMNQFKDV FLGADKRAYN 70 80 90 100 110 120 RATTSQRCVR AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKQDAIKF AWGFLTEVLQ 130 140 150 160 170 180 LPKERLLVTV YATDDEAFDI WEKEVGVPAD RIVRIGDNKG APYASDNFWA MGDTGPCGPC 190 200 210 220 230 240 TEIFYDHGDH IWGGRPGSPE EDGDRFIEIW NVVFMQFNRQ ADGTMEPLPR PSVDTGMGLE 250 260 270 280 290 300 RISAIMQGVH SNYEIDIFQA LIKKAAEIVG TSDLSNQSLR VIADHIRSCA FLVADGVMPS 310 320 330 340 350 360 NEGRGYVLRR IIRRAVRHGR KLGATEVFFY KLAAELAAQM KDVAAELIAQ LPLVERVLRI 370 380 390 400 410 420 EEEQFVRTLD RGLLLLEDVL ANLGDAKVIP GEVVFKLYDT YGFPADLTAD VVREREIGID 430 440 450 460 470 480 EEGFKAEMEK QRARAKEASS FGVNYNEMLK LDFETPFTGY KTLTERTRVV GIYKGVDEVS 490 500 510 520 530 540 GLIAGDEAVI VLEQTPFYAE SGGQIGDSGT LKVDDGIFAV TDTVKAGKAI IHKGYMELGT 550 560 570 580 590 600 LEKGAEVEAV VDGERRQAIA LNHSVTHLLH AALRQALGEH VTQKGSLVGA ERMRFDFSHF 610 620 630 640 650 660 EGMTMATIRR VEELVNNQIR ANHEITTQLM DLEAAKSAGA MALFGEKYED DVRVVRMGDY 670 680 690 700 710 720 STELCGGTHA KRTGDIGFFK IIAESGIAAG VRRIEAVTGK AAIDFMHQVG EQIEEAAALV 730 740 750 760 770 780 KGDQFCIADK VRQVLDKAKM MERELEQLKA KLAAQAGNDL LGQVIEIKGQ KVLVAALEGA 790 800 810 820 830 840 DPKSLRGMLD ELKNQMKSGV VLLATSGEGK VNLIAGVTND LTGKVKAGEL VNLVAQQVGG 850 860 870 KGGGRPDMAQ AGGTQPEAVP AALQSVHSWL EERL

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK39700.1.
RefSeq	YP_858162.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KPG1.
Genome annotation databases
GeneID	4488540.
GenomeReviews	Gene locus AHA_3714 in contig CP000462_GR.
KEGG	aha:AHA_3714.
TIGR	AHA_3714.
Phylogenomic databases
OMA	GEFVIKL.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AERHH

Accession

Primary (citable) accession number: A0KPG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)