Reviewed, UniProtKB/Swiss-Prot A0JX88 (SYA_ARTS2)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Arth_2278

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347493

Sequences

	Sequence		Length	Mass (Da)
	A0JX88-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: BFC22B2487E21D5B	FASTA	892	96,049
	10 20 30 40 50 60 MKSQEITKRW IDFFVSKGHT AVPSASLVSS DPSLLFTVAG MVPFIPYLTA REEAPYNRAT 70 80 90 100 110 120 SVQKCIRTGD IEEVGKTARH GTFFQMCGNF SFGDYFKEDA IKFAWELLTT SVDDGGYGLP 130 140 150 160 170 180 PEKLWVTVYE EDDEAEQLWL KNTGMPAERI QRMGKADNYW STGQPGPAGP CSEIYYDRGP 190 200 210 220 230 240 SYGVEGGPIA DENRYVEIWN LVFMQYQIDN VRSKVDFDIT GELPKKNIDT GLGMERLAMI 250 260 270 280 290 300 LQDVENMYET DQVRPVIDKA AALSGKEYTS AESADDPHHT DDVRMRVVAD HIRSALMLIA 310 320 330 340 350 360 DGVTPSNEGR GYVLRRLIRR AVRSMRLLGV EKACLPDLLP ASRDAMKGVY PVVETDFDRI 370 380 390 400 410 420 SRIAYAEEKA FLRTIASGTA RLEDAVKDSK AAGQPLSGAD AFALHDTYGF PIDLTLEMAE 430 440 450 460 470 480 EAGLKVDEPE FRKLMLEQRQ RAQADAKGKK GSHADLSAFQ ELLAEGETVF TGYTELAGES 490 500 510 520 530 540 KVRGILSGGR KVSQASTGEE IELVLAETPF YAEAGGQAAD TGLITGDGFV VEVLDVQRPV 550 560 570 580 590 600 KGLSVHKAIV REGEIGADSL VQAAVDRERR HSAEQAHTGT HIVHAALHQI LGPEALQRGS 610 620 630 640 650 660 YNKAGYLRFD FAWGEGLSAA TRSEIEEVSN LAIRNNFQVE TKVMALAEAK ALGAMALFGE 670 680 690 700 710 720 NYGNEVRVVE IDGAWSRELC GGTHVANTSL IGSLSLLGDQ SVGSGNRRVE AFVGMEAFRH 730 740 750 760 770 780 LAAERALVTE LTEMLKVPSG LLADRIATTL TKLKTVEKEL ERLRKEQLTA AAAQLVGTAK 790 800 810 820 830 840 DAAGVKVIAH DAGQVSGADD LRGLALDLRT RLGSEAAAVA VAGVSNDRPV ILVATNEAAR 850 860 870 880 890 AAGVKAGALV RLAAGILGGG GGGKDDVAQG GGTDAAKVPA ALTAVVDAIT RR

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK03658.1.
RefSeq	YP_831758.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JX88.
Genome annotation databases
GeneID	4445321.
GenomeReviews	Gene locus Arth_2278 in contig CP000454_GR.
KEGG	art:Arth_2278.
TIGR	Arth_2278.
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ARTS2

Accession

Primary (citable) accession number: A0JX88

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)